Protein Info for Dshi_3515 in Dinoroseobacter shibae DFL-12

Annotation: O-methyltransferase family 2 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 transmembrane" amino acids 20 to 39 (20 residues), see Phobius details amino acids 54 to 73 (20 residues), see Phobius details PF16864: Dimerisation2" amino acids 54 to 121 (68 residues), 35 bits, see alignment E=1.7e-12 PF00891: Methyltransf_2" amino acids 147 to 353 (207 residues), 150 bits, see alignment E=9e-48 PF13489: Methyltransf_23" amino acids 198 to 355 (158 residues), 37.6 bits, see alignment E=2.7e-13

Best Hits

Swiss-Prot: 53% identical to CRTF_RHOCB: Demethylspheroidene O-methyltransferase (crtF) from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)

KEGG orthology group: K09846, hydroxyneurosporene methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to dsh:Dshi_3515)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQ08 at UniProt or InterPro

Protein Sequence (376 amino acids)

>Dshi_3515 O-methyltransferase family 2 (RefSeq) (Dinoroseobacter shibae DFL-12)
MSMSPGTYLRFSFLPEKNAFYRWWVRLAGSSAFQSWASAMPGTRRLARKDGEALFDLVSG
FVYSQVLQAFVTLDMPALLAREPLPAWRLAARTRMEPRQMEVLCQAAASLKLLKRRRDGS
YQLGRLGAALPGVPGLAEMIRHHDVLYRDLQDPMKFLSGTQETELARFWPYVFGQAGTND
PEIAERYSRLMADSQGLVAEETLRTIDFDGVHRLMDVGGGTGAFLRAVREKHPDLRLRLY
DLPAVIEGAELPPGADKTGGSFRDAPALPKGADAISLVRVLYDHADATVADLLRKVFAAL
PADGRVVVSEPMAGGDQPTRTGDAYFAFYTMAMQTGRARSAQEIADHLTRAGFVDVVCHT
TYRPFVTGVVSGRKPA