Protein Info for Dshi_3474 in Dinoroseobacter shibae DFL-12

Annotation: fumarate lyase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 95 to 286 (192 residues), 93.8 bits, E=7.1e-31

Best Hits

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 100% identity to dsh:Dshi_3474)

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• aromatic compounds degradation via β-ketoadipate (6/9 steps found)
• toluene degradation III (aerobic) (via p-cresol) (6/11 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (13/35 steps found)
• superpathway of aerobic toluene degradation (7/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.5.1.2

Sequence Analysis Tools

See A8LPE2 at UniProt or InterPro

Protein Sequence (348 amino acids)

>Dshi_3474 fumarate lyase (RefSeq) (Dinoroseobacter shibae DFL-12)
MAAGGQNRLFDGLFADPEIAALFSAETMFAHFRHYEMALTEAQGAVGRVKDAARIAARIA
KFEIAPDAISDRVTQDGVPVPAYVAALEAALGVDAAAVHLDATSQDLMDTSLALSLRRLS
EVLAARLDRVIVALDGLQAAQGARTLMGRTRMQAALPIPVATRLEAWQTPLLRARDRFPE
ARAGVEQLQFGGPVGQRSPQMAAIAERLAKALDLPPPGPVWHSTRDGVVGYGTWLALVTG
SLGKMGQDIALMSQQGLDDARLSGGGTSSAMPHKQNPIAAETLVTLARFNAVQIGGLHQA
MVHEQERSGAAWALEWMILPQICEATGAALRHAAALLDSIETLGKKPV