Protein Info for Dshi_3194 in Dinoroseobacter shibae DFL-12

Annotation: Nitrous-oxide reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 signal peptide" amino acids 1 to 44 (44 residues), see Phobius details TIGR04244: nitrous-oxide reductase, TAT-dependent" amino acids 10 to 643 (634 residues), 1136.3 bits, see alignment E=0 PF18793: nos_propeller_2" amino acids 183 to 252 (70 residues), 119.5 bits, see alignment E=9.3e-39 PF18764: nos_propeller" amino acids 430 to 500 (71 residues), 100.5 bits, see alignment E=5.8e-33 PF13473: Cupredoxin_1" amino acids 553 to 641 (89 residues), 28.5 bits, see alignment E=1.9e-10

Best Hits

Swiss-Prot: 73% identical to NOSZ_PARDE: Nitrous-oxide reductase (nosZ) from Paracoccus denitrificans

KEGG orthology group: K00376, nitrous-oxide reductase [EC: 1.7.2.4] (inferred from 100% identity to dsh:Dshi_3194)

Predicted SEED Role

"Nitrous-oxide reductase (EC 1.7.99.6)" in subsystem Denitrification (EC 1.7.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.4 or 1.7.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LM18 at UniProt or InterPro

Protein Sequence (647 amino acids)

>Dshi_3194 Nitrous-oxide reductase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTDKTPKGGISRRGLLGATAGGAALAGTYGGRAMLAGGALGAATAASTTKASAAAESTHV
APGQLDEYYGFWSSGQTGEVRIIGVPSMRELMRIPVFNICSATGWGITNESKKILTEGLT
EKTKKYLAANGLEFPRNGDLHHVHMSFTEGKYDGRFLFMNDKANTRVARIRCDVMKCDKI
IEIPNAKGIHGLRPQKWPRTNYVFANGEDEVPIVNDGQILDKPEEYTNFYTALNADEMTV
AWQVMVTGNLDNTDADYEGKYAFSTSYNSEMGMTLADMTESDMDHVVVFNIAEIEKGIAE
GDYIELNGVKVIDGRKGSNKKYTRYIPIPNNPHGCNMAPDKVHLCIGGKLSPTVSVIDVR
KLDALFEEDADPRSAVVAEPELGLGPLHTCFDGKGRAYTTLFLDSQVVQWDIAKAVEAYG
GADVNPILSKTDVHYQPGHNSTAEGETLDASDKFYITMNKFSKDRFLNVGPLKPENEQVF
VIDDTKLTLVHDGPTFAEPHDSIIVHPSKVNPVSVWDRNDPMWAETRRQAEADGIDLDDW
QEEVIRDGNKVRVYMTSQAPNFSLETFTVTEGDEVTVTVTNLDDIDDLTHGFTMGNHGVA
MELGPQATASVTFTAAQPGVYWYYCQWFCHALHMEMRGRMFVEPRDA