Protein Info for Dshi_3094 in Dinoroseobacter shibae DFL-12
Annotation: dihydroorotase, multifunctional complex type (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 39% identical to PYRC_SYNAS: Dihydroorotase (pyrC) from Syntrophus aciditrophicus (strain SB)
KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 100% identity to dsh:Dshi_3094)Predicted SEED Role
"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
- UMP biosynthesis II (6/6 steps found)
- superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
- UMP biosynthesis I (5/6 steps found)
- UMP biosynthesis III (5/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.2.3
Use Curated BLAST to search for 3.5.2.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LL78 at UniProt or InterPro
Protein Sequence (432 amino acids)
>Dshi_3094 dihydroorotase, multifunctional complex type (RefSeq) (Dinoroseobacter shibae DFL-12) MAPAKPLALIHARIVDPGTGELREGGLLLRNGVIAAHLGPAERLPADTEVLDCGGKCLAP GIVDIGVKVGEPGERHKESFRTAGRAAAAGGVTTMVTRADTTPAIDSPETLEFAARRAAE ASPVRLAPMAALTKGRQGREMVEIGFLQDAGAVAFSDGDHVVADPKVFQRCLAYATSLGA LVMAHPQDPGLSAGAAVTSGKFATLRGLPAVSPMAERMGLERDLALVEMTGARYHADQIT TARALPSLRRAREIGLDVTAGISIHHLTLNEFDVGDYRTFFKVKPPLRAETDRVAMVAAV AAGEIDIISSMHTPQDEESKRLPFEEAASGAVGLETLLPAALRLVHSGELTLPQLWRALS LNPANRLGLPQGRLDPGSPADLVLFDPDAPFVLDRTMLHSKSKNTPFDGARLQGRVLRTY VDGRPVHVRDGH