Protein Info for Dshi_3044 in Dinoroseobacter shibae DFL-12

Annotation: Feruloyl esterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF07519: Tannase" amino acids 57 to 474 (418 residues), 329 bits, see alignment E=5.8e-102 PF00326: Peptidase_S9" amino acids 131 to 217 (87 residues), 27.1 bits, see alignment E=2.8e-10

Best Hits

KEGG orthology group: K09252, feruloyl esterase [EC: 3.1.1.73] (inferred from 100% identity to dsh:Dshi_3044)

Predicted SEED Role

"Chlorogenate esterase" in subsystem Phenylpropanoid compound degradation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LL28 at UniProt or InterPro

Protein Sequence (476 amino acids)

>Dshi_3044 Feruloyl esterase (RefSeq) (Dinoroseobacter shibae DFL-12)
MRAAIFLSTVLLGASASAALATDAATCEGLRNTVFSGGYVTSARVIPAAETLPEYCEVRA
TALPAISIEVRLPLAGWNGKYYQAGCGGFCGILGRADAGAGWINAMRPGLERGYATATSD
SGHHGLSVVDAAWADNNPHAERDWGYRSISETHRVAQVMLEAFYGSGSEQEIFQGCSTGG
RMAHMAAQRFPELFDGIISGAPAMNYTDLVGTKMSYLMQVNLDADGNPILGPGKDGLIGD
AVMAQCDAVDGAEDGVIADPRACEVDLSGLQCTGAAGDDCLTEAELDVLAKWRQGPVNAA
GEQLYPGGIPEGSEPFWWLWLTGNADGGGRLVSAFADNFGAYMAFDQDPGHGWTAADFDF
ETDPARMSRSAGFYNADSPDLSAFRAAGGKMIVWHGWADAIVTPYKTVDWYEKAAEAAGG
EETLAENVALFMVPGLDHCGILAGPSGLTQAGLDPMTPLEAWLADGTAPTSILSAN