Protein Info for Dshi_2871 in Dinoroseobacter shibae DFL-12

Annotation: MaoC domain protein dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF19315: MC_hydratase
  • amino acids 5 to 311 (307 residues), 61.9 bits, E=6e-21
• PF13452: MaoC_dehydrat_N
  • amino acids 90 to 140 (51 residues), 22.4 bits, 1.2e-08

Best Hits

Swiss-Prot: 74% identical to MCH_RHOS4: Mesaconyl-CoA hydratase (mch) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K14449, mesaconyl-CoA hydratase (inferred from 100% identity to dsh:Dshi_2871)

MetaCyc: 74% identical to mesaconyl-CoA hydratase (Cereibacter sphaeroides)
RXN-8960 [EC: 4.2.1.148]

Predicted SEED Role

"Mesaconyl-CoA hydratase"

MetaCyc Pathways

• methylaspartate cycle (14/19 steps found)
• ethylmalonyl-CoA pathway (8/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• glyoxylate assimilation (8/13 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (7/14 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.148

Sequence Analysis Tools

See A8LJK1 at UniProt or InterPro

Protein Sequence (343 amino acids)

>Dshi_2871 MaoC domain protein dehydratase (RefSeq) (Dinoroseobacter shibae DFL-12)
MAKTNPGRFFEDYRVGQVIEHAVPRTVSGGERALYHALYPARHAIYSSDEFAKLCGLAES
PMDDLIAFHTVFGKSVPDVSLNAVANLGYAEGRFHRPTWRGDTLRARSEVIGLKQNSSGK
TGVVYVRTEGRNQRGELVLDYVRWVMVRKNDLAAPAPDTVVPELAAAVDPATLLVPEGLT
FADYDTVLSGEPHRLRDYAVGEIIDHVDGVTIEEAEHMMATRLWQNTSKTHFDVTPRADG
KRLIYGGHVMSMARALSFNGLANAQMIVAINGGSHANPCFAGDTVRAWSEVLDVAETGAP
GVGALRLRLVATKDGSVGALKGADGKYLPGVLLDLDYWALMPQ