Protein Info for Dshi_2773 in Dinoroseobacter shibae DFL-12

Annotation: amidohydrolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01979: Amidohydro_1
  • amino acids 55 to 417 (363 residues), 192 bits, E=1.8e-60
• PF07969: Amidohydro_3
  • amino acids 194 to 420 (227 residues), 49.9 bits, E=3.7e-17

Best Hits

Swiss-Prot: 46% identical to OXODE_PSEAE: 8-oxoguanine deaminase (PA0142) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03382, hydroxyatrazine ethylaminohydrolase [EC: 3.5.99.3] (inferred from 100% identity to dsh:Dshi_2773)

MetaCyc: 46% identical to 8-oxoguanine deaminase monomer (Pseudomonas aeruginosa)
RXN-11455 [EC: 3.5.4.32]

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3)" (EC 3.5.4.3, EC 3.5.99.3)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• superpathway of purines degradation in plants (13/18 steps found)
• drosopterin and aurodrosopterin biosynthesis (4/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Atrazine degradation
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3

Use Curated BLAST to search for 3.5.4.3 or 3.5.4.32 or 3.5.99.3

Sequence Analysis Tools

See A8LJ11 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Dshi_2773 amidohydrolase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTEILVRNARVLVTMDDARRELPECDLRLRDGVIAEIGPGLATDGAEVIDAAGCVVTPGL
INTHHHLFQTLTRGVPAAQDALLFGWLKALYPIWARMGPEDMRVSAEVGLAELALTGCTL
SSDHLYLYPNGVRLEDTIEAAANIGLRFHPTRGAMSIGESAGGLPPDALVEDEAAILEDC
IRVIDAFHDPSPGAMVQVGVAPCSPFSVSRELMRDAALLARDKGVRLHTHLAENAEDIAY
SEAQFGCRPGQYAEDLGWTGPDVWHAHCVKLDAAEIELFARSRTGVAHCPCSNCRLGSGI
APVRAMRDAGVPVALGVDGSASNDAGSLIAEARMALLLQRVQTGADAMAAREMLEIATRG
GAQVLGRDDLGQLALGKRADLAIWDVSGLEAAGAWDPVAALLLSGPGRVRDLLVEGRAVV
RAGQLVHRDARALAAEQARRLARLMA