Protein Info for Dshi_2695 in Dinoroseobacter shibae DFL-12

Annotation: putative ureashort-chain amide or branched-chain amino acid uptake ABC transporter periplasmic solute-binding protein precursor (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 signal peptide" amino acids 1 to 50 (50 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 53 to 402 (350 residues), 238.2 bits, see alignment E=2.2e-74 TIGR03407: urea ABC transporter, urea binding protein" amino acids 54 to 419 (366 residues), 556.3 bits, see alignment E=1.3e-171 PF13433: Peripla_BP_5" amino acids 54 to 422 (369 residues), 553.3 bits, see alignment E=2.6e-170

Best Hits

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 100% identity to dsh:Dshi_2695)

Predicted SEED Role

"Urea ABC transporter, substrate binding protein UrtA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LII7 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Dshi_2695 putative ureashort-chain amide or branched-chain amino acid uptake ABC transporter periplasmic solute-binding protein precursor (RefSeq) (Dinoroseobacter shibae DFL-12)
MQIPAPVQSAGPPCPPATRSLGNKRMKFFNKSLMAAGVATALLSTTALAQEDTIKVGILH
SLSGTMAISETTLKDVMLMLIEEQNKKGGINGQMLEAVVVDPASDWPLFAEKARELIEGE
GVDVVFGCWTSVSRKSVLPVFEELNSILFYPVQYEGEESQRNVFYTGAAPNQQAIPAVDY
LMNEEGVERWVLAGTDYVYPRTTNKILEAYLQSKGVAPEDIMINYTPFGHSDWQTIVSDI
ATFGSAGKKTAVVSTINGDANVPFYKELGNQGISAEDIPVVAFSVGEEELAGLDTEPLVG
HLAAWNYFMSVDTPENDAFIDTWIEFIGDENEVTNDPMEAHYIGFNMWVKAVEAAGTTDP
DAVIDSIVGVSVPNLTGGLSTMLPNHHITKPVLVGEIQDDGQFDVVWETPGLVRGDAWSD
HLPESAPLISNWLPPMSCGNFNTETGTCGGGS