Protein Info for Dshi_2471 in Dinoroseobacter shibae DFL-12

Annotation: UDP-N-acetylmuramyl-tripeptide synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase
  • amino acids 21 to 483 (463 residues), 458.6 bits, E=1.3e-141
• PF01225: Mur_ligase
  • amino acids 22 to 102 (81 residues), 39.7 bits, E=7.7e-14
• PF08245: Mur_ligase_M
  • amino acids 112 to 314 (203 residues), 173.5 bits, E=8.1e-55
• PF02875: Mur_ligase_C
  • amino acids 335 to 420 (86 residues), 85.6 bits, E=3.7e-28

Best Hits

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to dsh:Dshi_2471)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

See A8LSB2 at UniProt or InterPro

Protein Sequence (493 amino acids)

>Dshi_2471 UDP-N-acetylmuramyl-tripeptide synthetase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSVGYGPKSLAELGFAERGRPVTGLALDSRQVKEGYLFAGLPGSRVHGAEFIQYALRMGA
GAILTDAEGARIARDVLDGAEVTLIVAEDPRQAFSAAAALWYGAQPATMVGVTGTNGKTS
VASFTRQLWQGLGHAAANIGTTGIEGDFSADLAHTTPDPLTLHAALAEMAAQGITHAAME
ASSHGLDQRRLDGVRLVAAGFTNFSQDHLDYHETFEAYFAAKAGLFSRVLADQGMAVINL
DDPKGADMMAIAHGAGAELLTVGTHKDAMLRLTAQRFDADGQEMRFTWKGVAHQARLNLV
GGFQASNVLVAMGLVMATGAQVGDLVPLLPRLATVRGRMQKVARRENGATVFVDYAHTPD
ALKTALRALRPHVLGRLVVVFGAGGDRDSGKRPLMGAAAAAAADVVFVTDDNPRSEDPAL
IRAQVRAGCPEATDVPDRAEAILRGVDALEPGDVLLIAGKGHETGQTVGDVVYPFDDAEQ
ASVAVAALEGRLA