Protein Info for Dshi_2423 in Dinoroseobacter shibae DFL-12

Annotation: Undecaprenyldiphospho-muramoylpentapeptidebeta-N-acetylglucosaminyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 96 to 116 (21 residues), see Phobius details PF03033: Glyco_transf_28" amino acids 8 to 144 (137 residues), 89 bits, see alignment E=3.1e-29 PF04101: Glyco_tran_28_C" amino acids 187 to 351 (165 residues), 123.1 bits, see alignment E=1.2e-39

Best Hits

Swiss-Prot: 73% identical to MURG_RHOS1: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 100% identity to dsh:Dshi_2423)

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LS64 at UniProt or InterPro

Protein Sequence (369 amino acids)

>Dshi_2423 Undecaprenyldiphospho-muramoylpentapeptidebeta-N-acetylglucosaminyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MGTAPLLVIAAGGTGGHMFPAQALAEAMLRKGWRVKLVTDARGARYTGGFPHVVEIVEKS
SATFARGGALAKVVVPVKIAAGVASALLDMLRDRPAMVVGFGGYPAIPAMAAATLLRLPR
MIHEQNGVLGRVNQLFAKHVHAIACGTWPTTLPEGVQGAPTGNPVRNAILERAGAPYIPP
GDWPLSLLVLGGSQGARILSDVVPTAVAGLAAPLRDRIRVAHQARAEDLDRVVAAYDAAG
IRAEVQTFFHDVPKRMSEAQLVISRSGASTVADLTVIGRPSILVPYAAAAADHQTANARG
LVEAGAAILIPESKLDPATLAEQIALILDNPDGAVQMAHAALRIGHPNATDRLVDLVDHL
AAPPTHVAA