Protein Info for Dshi_2410 in Dinoroseobacter shibae DFL-12

Annotation: O-succinylhomoserine sulfhydrylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01053: Cys_Met_Meta_PP
  • amino acids 8 to 389 (382 residues), 420.1 bits, E=1.5e-129
• TIGR01325: O-succinylhomoserine sulfhydrylase
  • amino acids 8 to 389 (382 residues), 564.3 bits, E=4.8e-174
• PF01212: Beta_elim_lyase
  • amino acids 46 to 279 (234 residues), 27.5 bits, E=4.8e-10
• PF00155: Aminotran_1_2
  • amino acids 55 to 239 (185 residues), 41.7 bits, E=2.3e-14
• PF01041: DegT_DnrJ_EryC1
  • amino acids 61 to 184 (124 residues), 23.8 bits, E=6.4e-09
• PF00266: Aminotran_5
  • amino acids 82 to 229 (148 residues), 40.5 bits, E=4.5e-14

Best Hits

Swiss-Prot: 53% identical to METZ_MYCTU: O-succinylhomoserine sulfhydrylase (metZ) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K10764, O-succinylhomoserine sulfhydrylase [EC: 2.5.1.-] (inferred from 100% identity to dsh:Dshi_2410)

MetaCyc: 50% identical to O-succinyl-L-homoserine sulfhydrylase (Pseudomonas aeruginosa PAO1)
Cystathionine gamma-synthase. [EC: 2.5.1.48]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)" in subsystem Methionine Biosynthesis (EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• aspartate superpathway (21/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carotenoid biosynthesis - General
• Cysteine metabolism
• Methionine metabolism
• Porphyrin and chlorophyll metabolism
• Riboflavin metabolism
• Selenoamino acid metabolism
• Sulfur metabolism
• Terpenoid biosynthesis
• Tryptophan metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-, 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.- or 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See A8LS51 at UniProt or InterPro

Protein Sequence (392 amino acids)

>Dshi_2410 O-succinylhomoserine sulfhydrylase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTEKQTRTKLVHAGTRRSQYGEVAEAIFLTQGFVYDTAEAAEARFIEAGADEFIYARYGN
PTVRMFEDRIAAVEGTEDAFATASGMAAVNGALASMLRAGDHVVSARALFGSCLYILEEV
LTRYGVEVTFVDGTDLAAWRAAIRPDTKAVFFETISNPTLEVIDIEAVAKLAHAVGALVI
VDNVFATPIFSRAKDQGADVIVYSATKHIDGQGRCLGGVICGTRDFVRKVAEPYLKHTGG
SLSPFNAWVMLKSMETLDLRVRAQAETAQALAETLEGHAALEKVIFPHLPSHPQYDLARA
QMAAGGTVLALDIKGGKAGAFRFLNALEIVTISNNLGDAKSIATHPATTTHQRLTPEQRA
GLGITDGLIRLSVGLEDAGDLLRDLQTALAAV