Protein Info for Dshi_2372 in Dinoroseobacter shibae DFL-12

Annotation: orotate phosphoribosyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00336: orotate phosphoribosyltransferase
  • amino acids 20 to 187 (168 residues), 108.7 bits, E=1.4e-35
• PF00156: Pribosyltran
  • amino acids 78 to 164 (87 residues), 33.9 bits, E=9.7e-13

Best Hits

Swiss-Prot: 100% identical to PYRE_DINSH: Orotate phosphoribosyltransferase (pyrE) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K00762, orotate phosphoribosyltransferase [EC: 2.4.2.10] (inferred from 100% identity to dsh:Dshi_2372)

Predicted SEED Role

"Orotate phosphoribosyltransferase (EC 2.4.2.10)" in subsystem De Novo Pyrimidine Synthesis (EC 2.4.2.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.10

Sequence Analysis Tools

See A8LRS7 at UniProt or InterPro

Protein Sequence (226 amino acids)

>Dshi_2372 orotate phosphoribosyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MIPNSFPDQAEMARLTAGMLLEIEAVHFNAREPFILASGLPSPTYIDCRKLISFPRIRST
LMDFLTVTVMREAGFEAFDNIAGGETAGIPFGALVAERLALPMTYVRKKPKGYGRDAQIE
GVMREGERVLLVEDLTTDGGSKLKFVEAIRKTGATCGHTAVIFSYGIFPETESSLAAEGV
KLHHLCTWWDVLAEARARGSFDPETLGAVETFLNDPRAWQEAHKRD