Protein Info for Dshi_2318 in Dinoroseobacter shibae DFL-12

Annotation: Deoxyribodipyrimidine photo-lyase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 PF00875: DNA_photolyase" amino acids 10 to 170 (161 residues), 161.9 bits, see alignment E=1.3e-51 PF03441: FAD_binding_7" amino acids 274 to 470 (197 residues), 229 bits, see alignment E=3.9e-72

Best Hits

KEGG orthology group: K01669, deoxyribodipyrimidine photo-lyase [EC: 4.1.99.3] (inferred from 100% identity to dsh:Dshi_2318)

Predicted SEED Role

"Deoxyribodipyrimidine photolyase (EC 4.1.99.3)" (EC 4.1.99.3)

Isozymes

Compare fitness of predicted isozymes for: 4.1.99.3

Use Curated BLAST to search for 4.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LRM3 at UniProt or InterPro

Protein Sequence (476 amino acids)

>Dshi_2318 Deoxyribodipyrimidine photo-lyase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSSQTDRAPVIWWVRRDLRLSDNRALVAAVETGAPVIPVFLCDPILDSLGAAPKWRFGLG
AAALDAALRDRGSRLIYRKGDALETLRALVSETGAETVYWSRAYDPDQVARDKAVKSGLQ
DDGVDARSFTGHLLIEPWTVETGSGGFYKVYTPFWKAVRDRAMPEPQPAPDRIPAPDNWP
ESDRLDYWALGAAMNRGAAIVAPHLAIGEDAARGRLGAFIAHRVGDYDRARDIPGIEGTS
RLSENLTYGEIGPATCWQAGQRALAEGKAGAETFLKELVWREFAYHLVWHTPHLIEKNWR
AEWDHFPWRDDNADAETWRRGRTGIRFVDAAMREMYVTGTMHNRGRMIVASYLTKHLMTH
WRVGQAWFDDCLVDWDPASNAMGWQWAAGSGPDAAPYFRVFNPDTQLDKFDRDQSYQRRF
IAEGQGTPPEDARAYFDAIPRSWGLSPDDAYPDPIVPLDEGRKRALDAYQARKDAA