Protein Info for Dshi_2311 in Dinoroseobacter shibae DFL-12

Annotation: delta-1-pyrroline-5-carboxylate dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1221 transmembrane" amino acids 690 to 701 (12 residues), see Phobius details PF14850: Pro_dh-DNA_bdg" amino acids 67 to 178 (112 residues), 146.5 bits, see alignment E=6.3e-47 PF01619: Pro_dh" amino acids 188 to 480 (293 residues), 351.4 bits, see alignment E=6.2e-109 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 516 to 1008 (493 residues), 630.7 bits, see alignment E=7.9e-194 PF00171: Aldedh" amino acids 566 to 1005 (440 residues), 364.3 bits, see alignment E=1.2e-112

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 100% identity to dsh:Dshi_2311)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88)" in subsystem Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.2.1.88, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LRL6 at UniProt or InterPro

Protein Sequence (1221 amino acids)

>Dshi_2311 delta-1-pyrroline-5-carboxylate dehydrogenase (RefSeq) (Dinoroseobacter shibae DFL-12)
MNRAFDISSQIDNPFRAQVRAHYTAEETALLKSLAARIKLSAHEREKAAAAGARYVTRVR
NETRPSMMEAFLAEYGLSTSEGVGLMCLAEALLRVPDADTIDDLIEDKVAPSNWGAHLGH
SSSSLVNASTWALMLTGKVLDEDPRGPARALRGLVKRLGEPVVRTAVGQSMKVLGRQFVL
GQTIEEGLKNARELEKKGFTYSYDMLGEAARTDADARRYHAAYAQAITAIARQATGDVRS
SPGISVKLSALHPRYEYTHRHSVMADLVPRAAALVKQAAQAGIGFNVDAEEQDRLDLSLD
VIEAMMSDPDLDGWDGFGVVVQAYGRRAGPVIETLYDMAERYDRKIMVRLVKGAYWDTEI
KLAQELGVERFPVFTRKNNTDVSYMACAQMLLDRRDRIYPQFATHNAHTCAAVLQMAGNA
RDCFEFQRLHGMGASLHQIVKETEGTRCRIYAPVGAHQDLLAYLVRRLLENGANSSFVNQ
IVDPDIPAEAISADPVSEMEKLGDQIPNPAIRQPSDLFAPDRRNSRGYRVNEPASILPLM
TAREAFAETTWHARPMLAGGRDPTGPTREVHSPADKTRLVGTVQEASAEDVACALDAAET
GFRDWSARPVSERADMLRKLADMYEDNIAELTAITTREAGKTVLDGIAEVREAVDFLRFY
ANEAERLEEEDPGRPRGIFVCISPWNFPLAIFTGQIAAALVMGNAVLAKPAEQTPIIAAR
AVQMMRDCGLPDAALQLLPGDGPMVGGPLTSDPRIAGVCFTGSTEVAMIIHKALAKNAGP
EAVLVAETGGLNAMIVDSTALHEQAVRDILISSFQSAGQRCSALRILYVQEDVHDKLMEM
LSGALDALVIGDSWNLDVDVSPVIDADAQSDILGYIDQHRKAGTLIKTLAAPDSGTYVTP
AIVKVGGIADMEREIFGPVLHVATFKANEIDQVVDAINARRYGLTFGLHTRIDDRVEQIV
ERIQVGNVYVNRNQIGAIVGSQPFGGEGLSGTGPKAGGPLYLTRFRKVGKSTSHPAPQGA
VLGKAALNTALSSLDARNWAARPDRVHILRMALSGSTGVVRRALSETAAFDMSPQTLPGP
TGESNRLSMVPRGTVLCLGPTPEIAMAQAVQALGAGCAVVIALPGSTPLSQPLSDAGAPV
VTLDGTVDCVTLTELTGIEVVAAAGASDWTRTLRVALSQRDGPIIPLIVDEIAPERYVLE
RHLCIDTTAAGGNAKLLAASS