Protein Info for Dshi_2278 in Dinoroseobacter shibae DFL-12

Annotation: molybdopterin oxidoreductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 890 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF18364: Molybdopterin_N" amino acids 44 to 86 (43 residues), 40.2 bits, see alignment 4.1e-14 PF00384: Molybdopterin" amino acids 90 to 548 (459 residues), 175.9 bits, see alignment E=1.9e-55 PF01568: Molydop_binding" amino acids 659 to 776 (118 residues), 81.8 bits, see alignment E=5.5e-27

Best Hits

KEGG orthology group: K07812, trimethylamine-N-oxide reductase (cytochrome c) 2 [EC: 1.7.2.3] (inferred from 100% identity to dsh:Dshi_2278)

Predicted SEED Role

"tungsten-containing formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LR91 at UniProt or InterPro

Protein Sequence (890 amino acids)

>Dshi_2278 molybdopterin oxidoreductase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSLTQPTRRAFIKGGAALFGSANLLGTTAFAALDPNSFDSRVFHATHYGPFEGVVRDGKL
VGLNTIMDIDARPTEMLTTGVIDRTYDKSRINYPMVRKSYLEGWETGDIKPELRGKEEYV
RVDWDTAWSLTAKALLDAATNHGNETIFSSSYGGWSNAGTFRPNVLQGRLMNLIGGCTVT
AGDWSAGASQVALPHIIGDMEVYSTQSAWETIRDNTEVFVLVGCDPIKNNRVEYRVADHG
MYAHWETIRDAGVKFISINPQKTASDDYLNAEWVKIVPNTDVALFCAMANHVLVMGLEDK
AYMDKYTVGSEKWVAYLKGETDGVEKTPEWAAQITGIAAEKIREMAELFAKSNTEIAGAW
SLQRAQHGEMTHWAIINFAALTGKIGKPGQGVGFSWHYGNGGMPASGKSNPAGLSQGRNF
VKTVCPASRITDMLENPGKEVFYNGNTHTYPDVKVVFNAGNNFMSHQQDTNRLIKALEKV
ETVVSVDVWWTAATRWCDIVLPAASTLEQDDISSGGTYSNDKVYAMKKVIEPVGESLPDY
EIFEGLADKLGLWMQFTDGEDKMYHIKLAYEKSSASAHTPFEEFWEKGFARQEVPEEARK
WTRHGDFYSDPEANPLHTTSGKIEMFCEDFAKLEIDDMPGMPIWMEKHEYLGNAKEGQLH
VVSPHPWFRLHSQMDQSENLRNLYKVQGREPVRINSEDAASRGIEDGDLVELYNDRGTVI
AGAVVSDEIMPGVVSIYEGAWPQLDSKGRCNSGLVNFLTSTQRSSGVAQATTANTTLCYM
RKCEDPEGPNMAYEKPPIIENYELAAIDEDKLGLDRIYDLTEKLLADLGPGEKIYFERCT
VCHAPREVTHFTQQQWRGITPSMFPRAGLEGDEAALVMDYLMENAADAPR