Protein Info for Dshi_2264 in Dinoroseobacter shibae DFL-12

Annotation: sarcosine oxidase, alpha subunit family (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 980 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 2 to 979 (978 residues), 1240.8 bits, see alignment E=0 PF13510: Fer2_4" amino acids 13 to 97 (85 residues), 82.4 bits, see alignment E=9.1e-27 PF07992: Pyr_redox_2" amino acids 167 to 287 (121 residues), 30.7 bits, see alignment E=1e-10 PF01134: GIDA" amino acids 167 to 200 (34 residues), 21.9 bits, see alignment (E = 3.8e-08) PF12831: FAD_oxidored" amino acids 167 to 235 (69 residues), 38.3 bits, see alignment 4.6e-13 PF00890: FAD_binding_2" amino acids 167 to 202 (36 residues), 30.7 bits, see alignment (E = 8.9e-11) PF13450: NAD_binding_8" amino acids 170 to 213 (44 residues), 30.5 bits, see alignment (E = 1.6e-10) PF17806: SO_alpha_A3" amino acids 498 to 582 (85 residues), 121.5 bits, see alignment E=6.9e-39 PF01571: GCV_T" amino acids 601 to 866 (266 residues), 259.5 bits, see alignment E=1.2e-80 PF08669: GCV_T_C" amino acids 886 to 972 (87 residues), 46.9 bits, see alignment E=9.5e-16

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to dsh:Dshi_2264)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LR77 at UniProt or InterPro

Protein Sequence (980 amino acids)

>Dshi_2264 sarcosine oxidase, alpha subunit family (RefSeq) (Dinoroseobacter shibae DFL-12)
MRLDGAGLIDRDTPLRFQFDDRWMGGFAGDTLASALMANGVKLVARSFKYHRPRGIWSAG
SEEPNALVQVGKFARQVPNVRATQAELFEGLYATSQNRWPSLTLDLLSLNDLAAPFLGAG
FYYKTFMWPRSFWEKLYEPAIRRAAGLGALSGQTNTEPYEKAYAHCDLLVIGAGPAGLMA
ALTAARAGADVILAEEGDRCGGRLLSEQEEVDGASGQDWVSRIVAELVTSGRVRVMTRTT
VTGAYDGGIYGALERVGLHLADPAPALPRECFWRISARRAILCAGASERPIAFPMNDRPG
ILSAAALRTYANRYRTVPGPRVALFCNNDDAYRTARDLVASGVEVAAVVDTRAETPEGDY
PVFREARVVDTRGRHGLRRITVDGPDGRRQIEVDALGVSGGWNPNLHLTCHMYGRPTWNP
ALHAFVPTPGAVPGLDVAGAAAGVFSTHGALKSGVFRARKALESLELAPSSMPIPQADDA
PYVLSPIYAVDGAKGARGRAWLDFQNDVTVKDVRQAASENMTSVEHMKRYTTQGMATDQG
RVSNVLALSVLADATGRGIPETGTTTFRPPYTPVSLAALGAGAQGKGFAPERLTTSHAGS
LARGAPMVEAGLWYRPSYFPRDGETTWREACDREVGHVRSAVGVCDVSTLGKIELQGPDA
GAFLDLVYANTFSTLKPGRVRYGVMLREDGHVMDDGTTACLGPGHYVMTTTTVAAHKVMQ
HLEFVQQCLVPTMRVRFTSVTENWAQFAVAGPQARALLNDLLDAPVRNANFPFMACGAVR
LGGVGGRLFRISFSGEHAYEVAIPARYGAALFEQLVARAEAMGGGAYGMEALNVLRIEKG
FVTHAEIHGRVTADDVGLGRMVSAKKDCIGKTASQRPGLTDPDREQLVGLRPCDGRTALL
AGAHLFEPGAEAVRVTDQGYVTSVCFSPTLQTPIGLGFLRRGRARHGQRVMMIDHLRGVT
TECEVTDPVFFDPDGGRARG