Protein Info for Dshi_2244 in Dinoroseobacter shibae DFL-12

Updated annotation (from data): DNA repair endonuclease mmcB (DUF1052)
Rationale: Conserved and specific phenotype: important for resisting cisplatin. Also see PMID:26162909 which suggests a role for a homologous protein from Caulobacter in creating substrates for translesion synthesis
Original annotation: protein of unknown function DUF1052 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 146 PF06319: MmcB-like" amino acids 11 to 140 (130 residues), 161.2 bits, see alignment E=7.6e-52

Best Hits

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_2244)

Predicted SEED Role

"Transcription elongation factor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LR57 at UniProt or InterPro

Protein Sequence (146 amino acids)

>Dshi_2244 DNA repair endonuclease mmcB (DUF1052) (Dinoroseobacter shibae DFL-12)
MTDLLQPGQRLARGVCRHLRSLDFACVEEFTPDRGLRVDVMALGPKGELWVVECKSSRAD
FTSDSKWQGYLDWCDRYFWAVDSAFPCDLLPENTGLILADAYDAEIQRMPDLHKLPAARR
RALTQRFARAAALRLQATRDPEMTLA