Protein Info for Dshi_2242 in Dinoroseobacter shibae DFL-12

Annotation: Histidinol dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00815: Histidinol_dh
  • amino acids 14 to 420 (407 residues), 445.1 bits, E=1.3e-137
• TIGR00069: histidinol dehydrogenase
  • amino acids 25 to 419 (395 residues), 430.3 bits, E=4.2e-133

Best Hits

Swiss-Prot: 66% identical to HISX2_RHILO: Histidinol dehydrogenase 2 (hisD2) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 100% identity to dsh:Dshi_2242)

MetaCyc: 47% identical to sulfopropanediol 3-dehydrogenase monomer (Cupriavidus pinatubonensis JMP134)
RXN-11727 [EC: 1.1.1.308]

Predicted SEED Role

"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• L-histidine biosynthesis (10/10 steps found)
• 3-sulfopropanediol degradation I (1/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Histidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.23

Use Curated BLAST to search for 1.1.1.23 or 1.1.1.308

Sequence Analysis Tools

See A8LR55 at UniProt or InterPro

Protein Sequence (441 amino acids)

>Dshi_2242 Histidinol dehydrogenase (RefSeq) (Dinoroseobacter shibae DFL-12)
MAEYLKRGKSETERAEDDARTQATVTGILQDISTRGDAAVRDLSEKFDGYTPQAFRLSPS
EIEAAMQKVTTREMGDIRFAQDQIRNFAQVQRASMTDVEVETIPGVILGHKNIPVNSVGC
YVPGGKFPMVASAHMSVITAKVAGVKRVVASAPPVKGAPHPAIVAAMHEGGADEILVLGG
VQAVGAMALGTESIAPVDMLVGPGNAFVAEAKRQLFGRVGIDLFAGPTETCVIADDTVDG
EMVATDLLGQAEHGYNSPAVLITCSRKLAEDTLSEIDRLLAILPTAETARVSWEDYGEVI
LCTDYDEMLRVADDVASEHVQVMTDRDDWFLDNMTNYGALFLGPRTNVANGDKVIGTNHT
LPTKKAGRYTGGLWVGKFLKTHSYQKVLTDEAAKLVGEYGSRLCMLEGFVGHAEQCNVRV
RRYGGINVPYGEGAPYRDAAE