Protein Info for Dshi_2192 in Dinoroseobacter shibae DFL-12

Annotation: excinuclease ABC, B subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 725 TIGR00631: excinuclease ABC subunit B" amino acids 25 to 676 (652 residues), 1007.2 bits, see alignment E=1.5e-307 PF04851: ResIII" amino acids 33 to 158 (126 residues), 47.7 bits, see alignment E=5.3e-16 PF00270: DEAD" amino acids 37 to 103 (67 residues), 25.8 bits, see alignment E=2.5e-09 PF17757: UvrB_inter" amino acids 181 to 269 (89 residues), 98.6 bits, see alignment E=5.3e-32 PF00271: Helicase_C" amino acids 455 to 565 (111 residues), 67.9 bits, see alignment E=2.7e-22 PF12344: UvrB" amino acids 572 to 613 (42 residues), 80.8 bits, see alignment 1.5e-26 PF02151: UVR" amino acids 645 to 678 (34 residues), 38.1 bits, see alignment (E = 2.7e-13)

Best Hits

Swiss-Prot: 71% identical to UVRB_ZYMMO: UvrABC system protein B (uvrB) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K03702, excinuclease ABC subunit B (inferred from 100% identity to dsh:Dshi_2192)

MetaCyc: 62% identical to UvrABC excision nuclease subunit B (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit B" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQR1 at UniProt or InterPro

Protein Sequence (725 amino acids)

>Dshi_2192 excinuclease ABC, B subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MNQPVLHASADDVRTRPKLEGGRMFRMATSFEPAGDQPTAIAELSEGVRNGERDQVLLGA
TGTGKTFTMAKVIEQTQRPAIILAPNKTLAAQLYGEFKGFFPDNAVEYFVSYYDYYQPEA
YVPRSDTYIEKESQINEQIDRMRHSATRALLERDDVIIVASVSCIYGIGSVETYEAMTQD
LVAGQSYDQRQIMADLVAQQYRRNDAGFQRGSFRVRGDSLEIWPAHMEDRALKFSFFGEE
LESITEFDPLTGEKADTLDRIRIYANSHYVTPRPTLQQAIVSIKKELRIRLDQLVAEGKL
LEAQRLEQRTNFDIEMLEATGVCNGIENYSRYLTGRAPGEPPPTLFEYIPDNAIVFADES
HVSVPQIGGMYKGDFRRKMTLAEHGFRLPSCMDNRPLKFEEWDAMRPQSVFVSATPAAWE
LEQAGGVFTEQVIRPTGLLDPQVEIRPVEMQVDDLLDEVRRVTADGFRTLVTTLTKRMAE
DLTEYMHEQGIKVRYMHSDIDTLERIEILRDLRLGAFDVLIGINLLREGLDIPECGLVAI
LDADKEGFLRSETSLVQTIGRAARNAEGRVIMYADRITGSMERALAETNRRRAKQIAYNE
EHGITPETVKKNVEDVMAGLYQGDVDMNRVTAKVEKPLHGGNLETVLEGLRADMRKAAEN
LEFEEAARLRDEVKRLEAVDLAIADDPLARQSAIEAASDAAVKAQGRSTAGRPGQRGGNV
KRRKK