Protein Info for Dshi_2068 in Dinoroseobacter shibae DFL-12

Annotation: methylthioadenosine phosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 5 to 242 (238 residues), 151.2 bits, E=1.6e-48
• TIGR01694: methylthioadenosine phosphorylase
  • amino acids 5 to 241 (237 residues), 298 bits, E=2.3e-93

Best Hits

Swiss-Prot: 78% identical to MTAP_RHOS4: S-methyl-5'-thioadenosine phosphorylase (mtnP) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K00772, 5'-methylthioadenosine phosphorylase [EC: 2.4.2.28] (inferred from 100% identity to dsh:Dshi_2068)

MetaCyc: 52% identical to 5'-fluoro-5'-deoxy-adenosine phosphorylase (Streptantibioticus cattleyicolor)
Purine-nucleoside phosphorylase. [EC: 2.4.2.1]

Predicted SEED Role

"5'-methylthioadenosine phosphorylase (EC 2.4.2.28)" in subsystem Methionine Salvage (EC 2.4.2.28)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• superpathway of purine deoxyribonucleosides degradation (6/7 steps found)
• ureide biosynthesis (6/7 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• S-methyl-5'-thioadenosine degradation II (1/1 steps found)
• 5'-deoxyadenosine degradation I (2/3 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• superpathway of guanine and guanosine salvage (2/3 steps found)
• nucleoside and nucleotide degradation (halobacteria) (2/6 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• nucleoside and nucleotide degradation (archaea) (3/10 steps found)
• L-methionine salvage cycle III (3/11 steps found)
• arsenic detoxification (mammals) (7/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Methionine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1

Use Curated BLAST to search for 2.4.2.1 or 2.4.2.28

Sequence Analysis Tools

See A8LPV1 at UniProt or InterPro

Protein Sequence (289 amino acids)

>Dshi_2068 methylthioadenosine phosphorylase (RefSeq) (Dinoroseobacter shibae DFL-12)
MQTYIGIIGGSGLYEIDGLEGATWTTVDSPWGTPSDAILTGTLDGVKMAFLPRHGRGHVH
SPSTVPYRANVDALKRLGVTDVISVSACGSFREEMAPGDFVIVDQFIDRTFAREKSFFGT
GCVAHVSVAHPTCPRLGAACLTAGRDAGITVHETGTYLAMEGPQFSTLAESKMYRESWGC
DVIGMTNMPEAKLAREAELCYASVAMITDYDSWHPDHGEVDVNEIIKTLMGNSEKAKGLV
ARLPKLLGADRAPCPHGCDRALDYAILTAPEKRDPALVAKLDAVAGRVL