Protein Info for Dshi_2062 in Dinoroseobacter shibae DFL-12

Annotation: PTSINtr with GAF domain, PtsP (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 PF01590: GAF" amino acids 27 to 158 (132 residues), 52.7 bits, see alignment E=2.1e-17 PF13492: GAF_3" amino acids 27 to 158 (132 residues), 27.6 bits, see alignment E=1e-09 PF13185: GAF_2" amino acids 28 to 158 (131 residues), 49.6 bits, see alignment E=1.5e-16 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 183 to 727 (545 residues), 378.2 bits, see alignment E=3.1e-117 PF05524: PEP-utilisers_N" amino acids 185 to 302 (118 residues), 75.6 bits, see alignment E=1.2e-24 PF00391: PEP-utilizers" amino acids 328 to 400 (73 residues), 67 bits, see alignment E=3e-22 PF02896: PEP-utilizers_C" amino acids 427 to 716 (290 residues), 271.9 bits, see alignment E=1.9e-84

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 100% identity to dsh:Dshi_2062)

Predicted SEED Role

No annotation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LPU5 at UniProt or InterPro

Protein Sequence (747 amino acids)

>Dshi_2062 PTSINtr with GAF domain, PtsP (RefSeq) (Dinoroseobacter shibae DFL-12)
MADSIETESRKLLRRLQATLAEAGAGQERLDKITSLIADSMRTEVCSIYLFRDPDTLELC
ATEGLNPEAVHKTRMRIGEGLVGRVARTSRAINTADAPSTKGFRYMPETGEEVYSSFLGV
PIQRLGETLGVLVVQSRAKREYSTDEIYALEVVAMVLAEMKELGAFTGEGAALAAPHQNP
IMIRGGVAQEGIAKGHVYLHEPRVVVTKLVSDDPEAELVRLRGAVDQLRVSVDEMLDIAP
KGDAEQLQILEAYRMFANSRGWMRRMEESIDSGLSAEAAVEKEQSAARSRMERVPDAYLR
ERLHDLDDLSNRLLRLLTGQGGETGAEIPADPILVARNIGPGELLDYGRALKGIILEEGS
VGSHAAIVARALAIPLIVHAKRITTEALNGDLVLVDGEQGIVHLRPDDRVLAHFTDKIAM
QAKAQERYASIRDKPAETLCGSVISLTMNAGLMADLPSLPSSGAEGVGLFRTELQFLTRS
KVPRRSELAEIYARVLDAADGKRVVFRTLDIGSDKVLPYMKPQDEPNPALGWRAIRVGLD
KPGVMRMQLQALIRGANGRPLTVMFPFIAQLEEFTRARDALLREIDREAALGRTLPESVE
IGAMLETPSLAFAPNSFFEMADFVSIGGNDLKQFFFAADRENERVRRRYDTLNVSFLSLI
ELIVSRCRDVGTPLSFCGEDAGRPIEALCFAAMGLRSLSMRPASIGPVKHLLRRSDLREA
RAVITQARASGAMTVRPAVMEWLRHQN