Protein Info for Dshi_1958 in Dinoroseobacter shibae DFL-12

Annotation: NAD-dependent epimerase/dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 880 PF05368: NmrA" amino acids 529 to 653 (125 residues), 39 bits, see alignment E=2.3e-13 PF01370: Epimerase" amino acids 529 to 758 (230 residues), 106 bits, see alignment E=6.3e-34 PF01073: 3Beta_HSD" amino acids 530 to 663 (134 residues), 45.5 bits, see alignment E=1.6e-15 PF16363: GDP_Man_Dehyd" amino acids 531 to 825 (295 residues), 65.1 bits, see alignment E=2.4e-21 PF13460: NAD_binding_10" amino acids 533 to 647 (115 residues), 42.8 bits, see alignment E=1.6e-14 PF07993: NAD_binding_4" amino acids 582 to 708 (127 residues), 44.1 bits, see alignment E=4.5e-15

Best Hits

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_1958)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LP17 at UniProt or InterPro

Protein Sequence (880 amino acids)

>Dshi_1958 NAD-dependent epimerase/dehydratase (RefSeq) (Dinoroseobacter shibae DFL-12)
MRKEGGSDGVDPLRGVRPHPEAGPALTAGNFRRLAKNGIGDPANAYVHGMEYFEGRLYCG
MTRNSFKLLNLFPPIDPPALDPWPVDVPPNVRDLDMQGQIWRWTPEDDSWEMAYRSPLIE
GKTGEEAPRDLGYRGMRVFQGKSDRKPNLYVSTMSTVLRGCAAHVLRSEDGLNFEPACEP
GIGNDNISTFRELVDFDGYLYAPPAGEGIQFNSNRTGVLKRSNDPRPGNWETACDLGFGD
KTNNGIFMMAKANDQLYAGTFNNYEGYQVWTTPRCGDGPLQWRRVIEKGAYRGPLSEIAM
GMVEFNGALYVGSSIQNGGYDRTNLVGPGAGEIIRIWPDDSWDLLIGMPRDTPQGFKYPL
SGIAPGFDNIFSGYTWRMCVHDGWLYVTTFDWSVFLQYAHRPSPTAKRLVDAVSFEQLAE
VGGGFELWRTKDGVNWQPVTINGLNNPYNYGGRTMVSTPHGLAVGTANVFGGKSPARFAS
GWQYVENPDGGTEILLGDHNIKPYETPAPVRSAEVADIAAPKRTRRCDIAITGCTGFIGT
NLIPKLLAKGYTIRALVLPGTGDVLPDSPQIELIEGGLGDTDALARLVEGARVVLHMAAR
LAGSCTLVELRETNVEGTHNLIRAVNAAGACARFVFCSSVAAYQNQFAEAEWPIHESSAL
RVDGGHDLSDYGMTKIAGENLARFYGRKFGYEHAILRFALVYGVGDPLVDPMVKTEAPKP
EFGKGQQGEQPRQFVHVTDAVEAILRACFEPGARNETFTVAGPDVITYRDMGRMTRIAIG
KASKDDLDPDRTRVWRRYVQQYDISKGKNKLKFYPSVPFAEGLREIVEAAQAKGELPRPG
QAPRLARPGVDRPQRVLTEGQGADAADLPQETRGAAISAE