Protein Info for Dshi_1851 in Dinoroseobacter shibae DFL-12

Annotation: aconitate hydratase 1 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 23 to 920 (898 residues), 1319.1 bits, E=0
• PF00330: Aconitase
  • amino acids 83 to 592 (510 residues), 540.8 bits, E=3.2e-166
• PF00694: Aconitase_C
  • amino acids 722 to 847 (126 residues), 138.2 bits, E=2.2e-44

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• methylaspartate cycle (14/19 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• mixed acid fermentation (10/16 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Sequence Analysis Tools

See A8LN80 at UniProt or InterPro

Protein Sequence (928 amino acids)

>Dshi_1851 aconitate hydratase 1 (RefSeq) (Dinoroseobacter shibae DFL-12)
MPITVGQDTAKTRKTLRVGDQSLAYYSIPAAETAGLGDFSKLPAALKVVLENMLRFEDGG
RTVSVDDIRAFADWADKGGQNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVGLGGDA
EKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNIERYTFLKWGQSAFANFRVVP
PGTGICHQVNLEYLAQAVWTDTDQSGQEVAYPDTLVGTDSHTTMVNGMAVLGWGVGGIEA
EAAMLGQPISMLIPEVVGFELTGEMMEGTTGTDLVLKVVEMLRAKGVVGKFVEFYGAGLD
HLPLADRATIANMAPEYGATCGFFPIDGETLRYMRTTGRDEARIALVEAYAKENGLWRGD
DYAPVYTDTLSLDMGTIVPAISGPKRPQDYVALDKAAETFRDYVTGQRPDWSADEEDKAE
WTDEGGAVAPRDIPGDRGKHKRARVRGADYTIHDGTIVIASITSCTNTSNPYVMIGAGLV
ARKARALGLTRKPWVKTSLAPGSQVVSAYLEAAGLQEDLDAIGFNLVGYGCTTCIGNSGP
IQEELSEAINDGDIIATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGDMNVDLTR
DPLGQDRDGNDVYLKDIWPSTKEIAELVEQTVTREAFQAKYADVFKGDEKWQSVETTDSL
TYDWPPSSTYVQNPPYFQGMSAEPGTISNIEGAKILAILGDMITTDHISPAGSFKESTPA
GRYLTERQVQPREFNSYGSRRGNHEVMMRGTFANIRIRNEMLDGVEGGYTLGPDGQQTSI
FEAAMAYQEMGTPLVIFGGEQYGAGSSRDWAAKGTSLLGVKAVIAESFERIHRSNLVGMG
VIPFEFTGGDTRKTLGLKGDETVSITGLDGKIVPLSEMPCTITYGDGTTREITLKCRIDT
EVEIEYIENGGVLHYVLRNLAKAPVAAE