Protein Info for Dshi_1775 in Dinoroseobacter shibae DFL-12

Annotation: (Glutamate--ammonia-ligase) adenylyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 936 PF03710: GlnE" amino acids 48 to 272 (225 residues), 169.6 bits, see alignment E=1.3e-53 amino acids 530 to 763 (234 residues), 178.3 bits, see alignment E=2.8e-56 PF08335: GlnD_UR_UTase" amino acids 297 to 439 (143 residues), 97.7 bits, see alignment E=9.4e-32 amino acids 801 to 885 (85 residues), 23.6 bits, see alignment E=6.7e-09

Best Hits

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to dsh:Dshi_1775)

Predicted SEED Role

"Glutamate-ammonia ligase adenylyltransferase (EC 2.7.7.42)" (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LMH2 at UniProt or InterPro

Protein Sequence (936 amino acids)

>Dshi_1775 (Glutamate--ammonia-ligase) adenylyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTFETRMTRLPLPFDPERGAAAVAAHCPEATGTLRDALVGMAGSSPYLEGLLAKEAAWFA
AALEDPEAAFAQILAEVPEIAPDQIKPGLRVAKRRVALLAGFADLAGVWPLEAVTENLTL
FADATMGAALRAALAPELRRGKVPGQGEADLPEAAGMVMLAMGKMGAFELNYSSDIDLIC
LFDQDRFAPDAFGEARAAFVRATRRAVGIMSDPTGDGYVFRTDLRLRPDPSVTPVCIAME
AAERYYESLGRTWERAAHIKARPAVGDLEAGAGYLARLTPFIWRKHLDFAAIEDAHDMRL
RIRAHKGLHGPVSLPGHDMKLGKGGIRDIEFFTQTRQIISGGRDPDLRVRGTVAGLAVLA
EKGWVRPEDAAVLSDHYRAHREVEHRVQMIADAQTHALPKDDDGFRRLACLTGEADVAGW
RRQLKDRLAEVAALTEPFFAPERSASSPPPEAVPEGAAEVMERWRSYPALRSTRALEIFG
RLRPEILSRLGRAARPDEALLNFDSFIKGLPAGVQLFSLFEANPSLIDLIVDICATAPLL
SRYLAANSGVLDAVIGGPFFADWPGEAALAAELEARLAPLDDYEHQLDAARRWQKEWHFR
VGVHHLRGLVDAQVAAEQYTDLAETVVSVLWPRVAAELSRRHGPPPGRGAVFVGMGSLGA
RTLTAQSDLDAIVVYDGAGVEASEGRRPLAPGPYYAKLTKAMITALTAPMAEGRLYEIDM
RLRPSGRQGPVATSWSAFQSYQESDAWTWEHLALTRARVIGGAGPESGALAEDLEAFRCA
LLRSKGPGRQAEVARDVADMRARLEAAKPAERPFEAKQGPGRLQDIELLGQAAALLSGAE
CADTRLQLAAGVAAGCLGPEEAAVLTGQLALLRQVQTSVRLLTDGALDPDAVGAGGQAFL
LRQTGRADMETLRADMAAGADAAAEVIVAVLTRWQG