Protein Info for Dshi_1769 in Dinoroseobacter shibae DFL-12

Annotation: 6-phosphogluconate dehydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 TIGR01196: phosphogluconate dehydratase" amino acids 7 to 599 (593 residues), 939.6 bits, see alignment E=3.1e-287 PF00920: ILVD_EDD_N" amino acids 68 to 381 (314 residues), 332.8 bits, see alignment E=2.2e-103 PF24877: ILV_EDD_C" amino acids 406 to 597 (192 residues), 166.1 bits, see alignment E=6.2e-53

Best Hits

Swiss-Prot: 62% identical to EDD_RHIME: Phosphogluconate dehydratase (edd) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to dsh:Dshi_1769)

MetaCyc: 57% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LMG6 at UniProt or InterPro

Protein Sequence (601 amino acids)

>Dshi_1769 6-phosphogluconate dehydratase (RefSeq) (Dinoroseobacter shibae DFL-12)
MALNRVISDVTARIEARSAEARSTYLDRMRRAAEDGPRRAHLSCGNQAHAYAAMGGDKEA
LVAERSANIGIVTAYNDMLSAHQPFKDYPDKIKEAARRAGATAQVAGGVPAMCDGVTQGQ
VGMELSLFSRDVIALATGVALSHNTFDAAAYLGVCDKIVPGLVIAAATFGYLPGVFVPAG
PMVSGLPNDQKAKVRQQFAAGEIGRDKLMEAEMASYHGPGTCTFYGTANSNQMLMEFMGL
HLPGASFVNPGTPLREALTAAAAERLAAITQLGNEYRPVCDILDAKAFVNGIVGLMATGG
STNLVIHLPAMARAAGVILDLQDFADISEATPLMARVYPNGLADVNHFHAAGGLAYMIGE
LLSEGLLHPDTKTIAGDGLADYAREPKLIDGVLRWEDGPRRSLNAKILRPASDGFAPSGG
LKELKGNLGRGVMKVSAVAPERHVIEARARVFEDQGAVKDAFKAGEFTEDTVVIVRFQGP
KANGMPELHALTPVLAVLQDRGLKVALVTDGRMSGASGKVPAAIHVAPEALDGGLMAKVR
TGDLVRVDAVAGVLEVLEPGVEDRAPAMPDLSGNSHGIGRELFDVFRTTVGPASTGAGVV
V