Protein Info for Dshi_1713 in Dinoroseobacter shibae DFL-12

Annotation: phosphoribosylformylglycinamidine synthase II (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 PF18072: FGAR-AT_linker" amino acids 18 to 55 (38 residues), 46.4 bits, see alignment 6.8e-16 TIGR01736: phosphoribosylformylglycinamidine synthase II" amino acids 20 to 704 (685 residues), 888.9 bits, see alignment E=1.3e-271 PF00586: AIRS" amino acids 76 to 190 (115 residues), 100 bits, see alignment E=1.6e-32 amino acids 438 to 554 (117 residues), 81.5 bits, see alignment E=9e-27 PF02769: AIRS_C" amino acids 204 to 354 (151 residues), 118.8 bits, see alignment E=3.8e-38 amino acids 566 to 697 (132 residues), 49.7 bits, see alignment E=7e-17

Best Hits

Swiss-Prot: 83% identical to PURL_RUEPO: Phosphoribosylformylglycinamidine synthase subunit PurL (purL) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 100% identity to dsh:Dshi_1713)

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.3

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLS7 at UniProt or InterPro

Protein Sequence (723 amino acids)

>Dshi_1713 phosphoribosylformylglycinamidine synthase II (RefSeq) (Dinoroseobacter shibae DFL-12)
MPASITEPAITPDLIAAHGLKPEEYARILEIIGREPSFTELGIFSAMWNEHCSYKSSKIH
LRKLPTEGPQVICGPGENAGVVDIGDGQAVVFKMESHNHPSYIEPYQGAATGVGGILRDV
FTMGARPIAAMNALSFGEVSHPKTRSLVAGVVEGIGGYGNCFGVPTVGGEVRFHPAYNGN
CLVNAFAAGLADADKIFYSAASGVGMPVVYLGAKTGRDGVGGATMASAEFDDTIEEKRPT
VQVGDPFTEKRLMEACLELMATGAVISIQDMGAAGLTCSAVEMGDKGGLGIRLDLEKVPV
RETAMTAYEMMLSESQERMLMVLNPALEAEAKAVFDKWDLDFAIVGETIAEDRFLIVHHG
ETKADLPLSKLASSAPEYDRPWVPTPEAAPLDPVPAVDPIDGLKALLASPNYARKHWVWE
QYDTQVMADTLRTPGLGAGIVRVHGTDKALAFTSDVTPRYVRANPVQGGRQAVAEAYRNL
IAVGARPLATTDNLNFGNPEKPEIMGQFVGALQGIGEACAALDMPIVSGNVSLYNETDGQ
GILPTPTIGAVGLIDHVDEAIMGQPRAGDILLLIGAGEGHLGQSALLAEVFNREEGDAPP
VDLDAERAAGELILEVREWIGACTDLSDGGLALAAFEMAEAAGLGVSLDEADMAQLFGED
QGRYIVACGFDAAEAIMVAAGRAGVPVASVGRFGGEQVSLGASSAPLAELSTLYRGAFAA
QLG