Protein Info for Dshi_1676 in Dinoroseobacter shibae DFL-12

Annotation: HAD-superfamily hydrolase, subfamily IA, variant 3 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 PF00702: Hydrolase" amino acids 2 to 175 (174 residues), 94.2 bits, see alignment E=2.9e-30 PF13419: HAD_2" amino acids 5 to 180 (176 residues), 97.7 bits, see alignment E=1.9e-31 PF12710: HAD" amino acids 6 to 149 (144 residues), 56 bits, see alignment E=1.5e-18 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 51 to 180 (130 residues), 62.3 bits, see alignment E=6.2e-21

Best Hits

Swiss-Prot: 39% identical to SGGP_ARATH: Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp (SGPP) from Arabidopsis thaliana

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_1676)

Predicted SEED Role

"Beta-phosphoglucomutase (EC 5.4.2.6)" in subsystem Maltose and Maltodextrin Utilization or N-Acetyl-Galactosamine and Galactosamine Utilization or Trehalose Uptake and Utilization (EC 5.4.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLP0 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Dshi_1676 HAD-superfamily hydrolase, subfamily IA, variant 3 (RefSeq) (Dinoroseobacter shibae DFL-12)
MIHALLFDLDGTLLASDPLHAEAFIALFAEHGRTIDTAFYNARIHGRLNAEIFAEFFPDA
DGAAMAEAKEAAFRDLLGPSASPMPGLTALLDRADAQGWRTAVVTNAPRINAEFMLKAIG
LADRFDTVVVSDECAAGKPDPAPYREALRRLDCAPRAARAFEDSPSGIRSAVAAGITTLG
LRSSLPDIALRAAGAAASLADFTDPTLEPYLQRQSGADP