Protein Info for Dshi_1642 in Dinoroseobacter shibae DFL-12

Annotation: alanyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 6 to 554 (549 residues), 731.2 bits, E=1.2e-223
• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 868 (863 residues), 971.1 bits, E=2.8e-296
• PF07973: tRNA_SAD
  • amino acids 652 to 707 (56 residues), 46.7 bits, 3.8e-16
• PF02272: DHHA1
  • amino acids 743 to 885 (143 residues), 78.9 bits, E=7.7e-26

Best Hits

Swiss-Prot: 100% identical to SYA_DINSH: Alanine--tRNA ligase (alaS) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 100% identity to dsh:Dshi_1642)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• superpathway of chorismate metabolism (38/59 steps found)
• ergotamine biosynthesis (4/12 steps found)
• enterobactin biosynthesis (3/11 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (2/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See A8LL20 at UniProt or InterPro

Protein Sequence (887 amino acids)

>Dshi_1642 alanyl-tRNA synthetase (RefSeq) (Dinoroseobacter shibae DFL-12)
MPSLNEIRSTFLNFFGGHGHEIVDSSPLVPRNDPTLMFTNAGMVQFKNVFTGLEHRDYNR
ATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEDAIPFAWDLITKEFGI
DKSRLLVTVYHTDDEAAEIWKKHAGLSDDRIIRIPTNDNFWMMGPTGPCGPCTEIFYDHG
DHIWGGPPGSPEEDGDRFIEIWNLVFMQFEQFEDGTREPLPKPSIDTGMGLERIGALLQG
SHDNYDTDLFKTLIEASAHATSVDPYGDKNVHHRVIADHLRSTSFLIADGVMPSNEGRGY
VLRRIMRRAMRHAHLLGAQDPVMYRLVPALVGQMGQAFPELGRAQALIEETLMLEETRFR
QTLDRGLKLLDEAVADVPEGGALPGETAFKLYDTYGFPLDLTQDALREKGRAVETEGFDA
AMADQKAKARAAWAGSGETADESIWFDLAETHGATEFLGYDTEVAEGQVLALVKDGAQVA
QLAEGETGWMVLNQTPFYAESGGQVGDSGGYLKSNGSGAIHDTQKRNGLFAHKVTPKAQP
LKAGDVLEMRVDPARRTAIRANHSATHLLHEALRQALGAHVAQRGSLNAADRLRFDFSHS
KALTLEELQKVEAEVNAYIRQNSPVETRIMTPDAARDLGAQALFGEKYGDEVRVVSMGRQ
SGSGKGVSGETYSLELCGGTHVKQTGDIGVFVTVGETASSSGVRRIEALTGEDAFAYLSA
QDYRLAEVAASLKAQAADVPERVRSLLDERKALQNEVAQLRRELAMAGGGQGTAAPEAEA
VAGVPFLAQSLSGVSGRDLPSLIDEHKARLGSGAILLIADTGGKAAVAAGVTADLTDRLS
AVDLVKAAVAELGGKGGGGRPDMAQGGGKDASKADAAIAAAKAVIGG