Protein Info for Dshi_1573 in Dinoroseobacter shibae DFL-12

Annotation: multi-sensor signal transduction histidine kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 767 transmembrane" amino acids 24 to 45 (22 residues), see Phobius details amino acids 57 to 76 (20 residues), see Phobius details amino acids 97 to 122 (26 residues), see Phobius details amino acids 301 to 323 (23 residues), see Phobius details PF19312: NtrY_N" amino acids 54 to 305 (252 residues), 105 bits, see alignment E=1.5e-33 PF00672: HAMP" amino acids 321 to 373 (53 residues), 55.5 bits, see alignment 1.7e-18 PF00989: PAS" amino acids 393 to 497 (105 residues), 37.9 bits, see alignment E=4.7e-13 PF08448: PAS_4" amino acids 399 to 500 (102 residues), 25.4 bits, see alignment E=4.1e-09 PF00512: HisKA" amino acids 510 to 576 (67 residues), 36.8 bits, see alignment E=9.8e-13 PF02518: HATPase_c" amino acids 620 to 737 (118 residues), 66.8 bits, see alignment E=6.7e-22

Best Hits

KEGG orthology group: K13598, two-component system, NtrC family, nitrogen regulation sensor histidine kinase NtrY [EC: 2.7.13.3] (inferred from 100% identity to dsh:Dshi_1573)

Predicted SEED Role

"Nitrogen regulation protein NtrY (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LKV1 at UniProt or InterPro

Protein Sequence (767 amino acids)

>Dshi_1573 multi-sensor signal transduction histidine kinase (RefSeq) (Dinoroseobacter shibae DFL-12)
MRAVTGRYLSRWVTRYVSLRNLQTWTTLGMVALGPILAIATFLVLGPLDQGQDATTLRVV
ILADFVYVLILAALVLQRVARMIAARRARSAGSRLHLRLTGVFAIVALVPTVLVAVFATI
TVNFGLEGWFSDRVQRVLGASLAAAEAYEEEQRVQLQIDTAALASYLDRARAERVFLSDG
DLRQLLAQGQSSVQRGLREAYIVNANGDIRIRGERSYLFDFERPTPDQIAASEQGTPIVI
EDWTNNEFRALVKLARHVDRYLYVSREVDGEILTLLDDTQETVQLFRQLESDRGQLLFDF
GLLYIGFAVILIIASIWLGLWFAERLSRPVGRLAGAAQRVGAGDLDVKVPEQQGDDEIAM
LGRLFNQMTRQLKAQREALLENTNQIERRRRLFDSVLGSVTAGVIGLDSRGRVDFMNRSA
TAMLALDGVTVSGRGIDEVVPEFEDLFDKLQGGLRDVAQQQVSLTRQGRQESLLVRIAKR
LNREDRLEGYVVAFDDVTDLATAQRMAAWGDVARRIAHEIKNPLTPIQLSAERMKRKFGP
RLGEDGPALVELSDVIIRQTADLRRIVDEFSKFARMPEPETAHLDLAQLVRDAVLLQQVA
QPDVTFVTDISPDPVPADLDATLMGQALTNLLKNAGEAIESRQTGKTGDTRPGEIRVSLR
NLGREAELTIADNGIGLPPDTAKLFEPYVTTRDKGTGLGLPIVRKIVEEHQGTLELRPAE
PFTPDTPPGAAAVILLPTRPPDPEAATQVDASRPEPDITPPKKTATA