Protein Info for Dshi_1476 in Dinoroseobacter shibae DFL-12

Annotation: DNA gyrase, A subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 913 TIGR01063: DNA gyrase, A subunit" amino acids 24 to 898 (875 residues), 1108.8 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 46 to 511 (466 residues), 543 bits, see alignment E=5.3e-167 PF03989: DNA_gyraseA_C" amino acids 543 to 590 (48 residues), 54.9 bits, see alignment 4.6e-19 amino acids 595 to 629 (35 residues), 21.9 bits, see alignment (E = 9.9e-09) amino acids 654 to 697 (44 residues), 16.2 bits, see alignment 5.6e-07 amino acids 700 to 747 (48 residues), 42.8 bits, see alignment 2.7e-15 amino acids 800 to 834 (35 residues), 29.4 bits, see alignment (E = 4.4e-11) amino acids 851 to 895 (45 residues), 30.6 bits, see alignment 1.8e-11

Best Hits

Swiss-Prot: 54% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 100% identity to dsh:Dshi_1476)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LK19 at UniProt or InterPro

Protein Sequence (913 amino acids)

>Dshi_1476 DNA gyrase, A subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MTDTPEPPENTDETPPGGGRIQETISIAEEMKSSYLDYAMSVIVSRAIPDLRDGLKPVHR
RILYAMHETGNTHEKAYRKSARPVGDVMGKYHPHGDSAIYDALVRMAQDFSMSLPLLDGQ
GNFGSMDGDNPAAMRYTEVRMDKPAAALLADIEKETVDFQDNYDGKDREPTVLPARFPNM
LVNGAGGIAVGMATNIPPHNLGEVIDATLALIETPDLEVEDLMQYIPAPDFPTGGQILGR
GGARKAYLEGRGSVIIRAKTRIEELRKDRYAIVLDEIPYQVNKSSMIEKIAELVREKRIE
GIAHVQDESDRVGVRVVIELKRDATPEVVLNQLYRFTPMQTSFGCNMLALNGGRPEQLTL
KDFLSSFISFREEVVARRTAYDLRKARERSHVLCGLAVAVSNVDEVVATIRASADAAEAR
EKLMTRRWPAADIADYIRLIDDPTHTMNEDGTYNLSETQARAILDLRLQRLTQIGVKEVT
DELQELAAKIQEYLDILRSRSRIMAIISDELREVKDQFAVPRRTEITDWSGDMEDEDLIE
REDMVVTVTSGGYIKRTPLVEFRAQRRGGKGLAGMATKEDDVVTTLFVANTHTPLLVFTT
DGMVYKLKTWRLPSGGRNARGKALVNVLPIDPGTSIAAIMPVDRAEEDWADLQIVFATSA
GDVRRNALSDFTNVMRNGKIAMKLPEGVSLVKARICSEEDDVMLVTALGRAIRFRTTDVR
VFKGRDSTGVRGVRLAEGDRVVSMAVIRHFVADPQERAAYLKMRRAVAGLSEEAESEDED
TVEAGQLSPERYAEMSAAEDLILTITEGGTGKISSSHDYPVRGRGGQGVTAMDKAMRGGP
LVACFPVEIDDQIMLATSTGQSIRCPVDGISFRSRAAGGVKVFNTAPGEQVVSVAYIADR
GEADGDDPAPEAG