Protein Info for Dshi_1475 in Dinoroseobacter shibae DFL-12

Annotation: gid protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 TIGR00137: tRNA:m(5)U-54 methyltransferase" amino acids 4 to 442 (439 residues), 549.1 bits, see alignment E=3.4e-169 PF01134: GIDA" amino acids 5 to 374 (370 residues), 328.5 bits, see alignment E=6.2e-102 PF13450: NAD_binding_8" amino acids 7 to 42 (36 residues), 26.4 bits, see alignment 6.8e-10

Best Hits

Swiss-Prot: 100% identical to TRMFO_DINSH: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (trmFO) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K04094, glucose inhibited division protein Gid (inferred from 100% identity to dsh:Dshi_1475)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LK18 at UniProt or InterPro

Protein Sequence (451 amino acids)

>Dshi_1475 gid protein (RefSeq) (Dinoroseobacter shibae DFL-12)
MTETLHIVGGGMAGSEAAWQAAQMGVSVVIHEMRPKVGTFAHKTGHLAEMVCSNSFRSDD
DEQNAVGLLHWEMRAAGGLIMEMADAHALPAGGALAVDREPFAESVTARLHEHPNIRVEG
TEITSLPAEGKWIIATGPLTSGALAEAIAAETGQESLAFFDAIAPILYFDSIDMTKAWMQ
SRYDKGETEEERTAYLNCPMDRDQYEAFIDALLAAEKTEFKPGETAGYFDGCLPIEVMAE
RGRETLRHGPMKPVGLTNPHQPDVKAHAVVQLRRDNALGTLYNIVGFQTKMKYGAQKEVL
RMIPGLEEARFARLGGIHRNTFINAPTLLDDQMRLHSRPNLRFAGQITGVEGYVESAAMG
LLAGRMAAAEILGTPLASPPQETAMGALIHHITGGAEAKTFQPMNVNFGLFPPLDGVRGG
RRGRKERYKGYTDRAKAAWQGWLGQTALAAE