Protein Info for Dshi_1370 in Dinoroseobacter shibae DFL-12

Annotation: dihydropyrimidinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 TIGR02033: dihydropyrimidinase" amino acids 4 to 451 (448 residues), 594.6 bits, see alignment E=6.8e-183 PF01979: Amidohydro_1" amino acids 47 to 418 (372 residues), 87.1 bits, see alignment E=1.5e-28

Best Hits

Swiss-Prot: 54% identical to HYDA_PSEAE: D-hydantoinase/dihydropyrimidinase (dht) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 100% identity to dsh:Dshi_1370)

MetaCyc: 49% identical to dihydropyrimidinase monomer (Rattus norvegicus)
Dihydropyrimidinase. [EC: 3.5.2.2]; 3.5.2.2 [EC: 3.5.2.2]; 3.5.2.2 [EC: 3.5.2.2]

Predicted SEED Role

"Dihydropyrimidinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism or Pyrimidine utilization (EC 3.5.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LJ90 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Dshi_1370 dihydropyrimidinase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSTVIKNGTVVTADLTYEAEVRVEGGKITEIGQGLSGDTVLDATGCYVMPGGIDPHTHLE
MPFMGTYSSDDFESGTRAALSGGTTMVVDFALPSPGQGLLDAIQMWDNKSTRANCDYSFH
MAITWWGEQVFNEMEAVTKRGINTFKHFMAYKGALMVNDDEMFASFRRCAELGALPLVHA
ENGDVVADLQQKFLAEGNTGPEAHAYSRPPAVEGEATNRAIMIADMAGVPVYIVHTSCEE
AHEAIRRARMQGKRVYGEPLIQHLTLDESEYAHPDWDHAARRVMSPPFRNKQHQDSLWNG
LASGSLSVVATDHCAFTTAQKRTGVGDFTQIPNGTGGLEDRMPMLWTHGVNTGRLTPNEF
VAVTSTNIAKILNCYPTKGAILVGADADIVVWDPEKEKTISADSQQSAIDYNVFEGKHVK
GLPRYTLTRGHVAVMDGEIRTQEGHGKFVERPPVTPVSKALSTWKELTAPRKVERTGIPA
SGV