Protein Info for Dshi_1327 in Dinoroseobacter shibae DFL-12

Annotation: proton-translocating NADH-quinone oxidoreductase, chain L (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 29 to 50 (22 residues), see Phobius details amino acids 79 to 98 (20 residues), see Phobius details amino acids 118 to 135 (18 residues), see Phobius details amino acids 141 to 160 (20 residues), see Phobius details amino acids 172 to 193 (22 residues), see Phobius details amino acids 224 to 246 (23 residues), see Phobius details amino acids 258 to 280 (23 residues), see Phobius details amino acids 288 to 309 (22 residues), see Phobius details amino acids 316 to 338 (23 residues), see Phobius details amino acids 344 to 364 (21 residues), see Phobius details amino acids 385 to 411 (27 residues), see Phobius details amino acids 431 to 455 (25 residues), see Phobius details amino acids 476 to 496 (21 residues), see Phobius details amino acids 559 to 578 (20 residues), see Phobius details amino acids 660 to 680 (21 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 3 to 679 (677 residues), 799.6 bits, see alignment E=1.4e-244 PF00662: Proton_antipo_N" amino acids 62 to 109 (48 residues), 65.3 bits, see alignment 3.7e-22 PF00361: Proton_antipo_M" amino acids 135 to 443 (309 residues), 292.1 bits, see alignment E=4.5e-91

Best Hits

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 100% identity to dsh:Dshi_1327)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LIV5 at UniProt or InterPro

Protein Sequence (685 amino acids)

>Dshi_1327 proton-translocating NADH-quinone oxidoreductase, chain L (RefSeq) (Dinoroseobacter shibae DFL-12)
MESIILFAPLVGALICGFGWKFIGEKAALWVSTGFVFLAAILSWFVFLTFDGTTEQIQVL
RWIESGSLAADWAIRMDRLTAIMLIVVNTVSALVHLYSFGYMDHDPQWREGETYKPRFFA
YLSFFTFAMLMLVTSDNLVQMFFGWEGVGVASYLLIGFYWRKPSAGAAAMKAFIVNRVGD
FGFLLGIFALFYLTDSVNLTDIFAAAPELAETQISFLWTDWNAANLIAFLLFIGAMGKSA
QLFLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVCRMSPVMEFAPQAMTFVTFVGATTA
FFAATVGLVQNDIKRVIAYSTCSQLGYMFVAAGVGAYPVAMFHLFTHAFFKAMLFLGAGS
VIHAMHHEQDMRNYGGLRKKIPYTFWAMMIGTLAITGVGIPLTGWIGFAGFASKDAVIES
AFAGTNAAHMYAFWMLVIAALMTSFYSWRLMFMTFYGTPRGDKHTHEHAHESPKVMLIPL
GVLALGSVFAGAIWYGSFFGHTDEVAKFYGIPYAEEQSEEAHADDGHHYVYAGQPGEGAV
YMAPENTVMADAHDVPKWVKVSPFVAMLIGLGLSYLFYIRKPSLPGTFAETLWPVYNFIY
NKWYFDEIYDAVFVKPSKAIGRFLWTKGDGATIDGGINGLAMGIIPFFTRLAGRAQSGYL
FHYAFAMVLGITILVTWMMIGGGAE