Protein Info for Dshi_1294 in Dinoroseobacter shibae DFL-12

Annotation: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 3 to 122 (120 residues), 31.7 bits, E=3.4e-11
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 435 (432 residues), 476.5 bits, E=4.4e-147
• PF12804: NTP_transf_3
  • amino acids 6 to 133 (128 residues), 67.3 bits, E=4.6e-22
• PF00483: NTP_transferase
  • amino acids 6 to 205 (200 residues), 34.2 bits, E=5.2e-12
• PF00132: Hexapep
  • amino acids 265 to 299 (35 residues), 29.8 bits, 8.8e-11
• PF14602: Hexapep_2
  • amino acids 378 to 412 (35 residues), 26.2 bits, 1.4e-09

Best Hits

Swiss-Prot: 100% identical to GLMU_DINSH: Bifunctional protein GlmU (glmU) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to dsh:Dshi_1294)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• peptidoglycan recycling I (7/14 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (5/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A8LIS2 at UniProt or InterPro

Protein Sequence (450 amino acids)

>Dshi_1294 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (RefSeq) (Dinoroseobacter shibae DFL-12)
MGVALIVLAAGQGSRMNSELPKVLHPLAGAPLLAHGLRAGAALDPARIVVVTGHGAAAVE
AATAQLAPEAICVRQTEQLGTGHAVAQTAQALAGFGGDALVLYGDTPFISPETLLAMQAA
RAAGADMVVLGFEAADPGRYGRLVTDGDALLRIVEAKDATPDELALDLCNSGVMMADAQV
LLRLVAGLSNDNASREYYLTDTIAAGRAEGLRARVVVCPEAETLGINTRTELAAAEQAFQ
ARARARALEDGVTLADPATTHFAVDTVIGRDATIGPQVVFGPGVTVESGAEIRAFSHLEG
CHVSRGARVGPFARLRPGAELAENTHVGNFVEIKNATLAQGAKVNHLSYIGDAAIGEASN
VGAGTITCNYDGVFKHRTEIGARSFIGSNTCLVAPVRVGDEAMTATGTVVTQDIPDGAMA
VGRTRQENKPGFARKFMTMLRARKSAKGAQ