Protein Info for Dshi_0996 in Dinoroseobacter shibae DFL-12

Annotation: NADH/Ubiquinone/plastoquinone (complex I) (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 34 to 56 (23 residues), see Phobius details amino acids 64 to 85 (22 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 120 to 139 (20 residues), see Phobius details amino acids 151 to 173 (23 residues), see Phobius details amino acids 194 to 218 (25 residues), see Phobius details amino acids 230 to 251 (22 residues), see Phobius details amino acids 257 to 277 (21 residues), see Phobius details amino acids 296 to 318 (23 residues), see Phobius details amino acids 350 to 371 (22 residues), see Phobius details amino acids 378 to 404 (27 residues), see Phobius details amino acids 409 to 424 (16 residues), see Phobius details amino acids 444 to 466 (23 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 115 to 332 (218 residues), 103.6 bits, see alignment E=6e-34

Best Hits

KEGG orthology group: K05577, NADH dehydrogenase I subunit 5 [EC: 1.6.5.3] (inferred from 100% identity to dsh:Dshi_0996)

Predicted SEED Role

"NADH dehydrogenase, subunit 5" in subsystem Respiratory Complex I

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LSC4 at UniProt or InterPro

Protein Sequence (501 amino acids)

>Dshi_0996 NADH/Ubiquinone/plastoquinone (complex I) (RefSeq) (Dinoroseobacter shibae DFL-12)
MVLSLIPFLIPILLAAAGAVAFLRPEQALRAAPVAGLAAFALAALAALGFAVAGAPAGGA
RLDLVSAVMLPLVAGIGWVVLRFSVTALRDEAEGPAFLGWIGLALAAVLTLVLAPTLWML
WGAWVALSLCVYKLFLTYPGRPMARAAARKYLLSAGLVTVLLGLASFGLGTGYGTTSIVA
IQAAAETGIAPAGLGWSAVALILAAVVASALFPLSGWLTEAMEAPTPFSALLHAGIVNAG
GVLCLIFADVLLLVPGALALLAMIGGASALIGALVMLTQPAIKTGLAWSTVSQMGFLMLQ
IGLALFPLALLHILAHALYKAHALLSAGTAVDTVTTARSAGPVAVPEVKAVALAFSLALG
IYVFGVFALGAADKAPQALALGAMLILGIAYLIAQGLAGAAPWALTGRLAAASALATVAY
LAFQAGAETLAVGHLPLPPVPGALDWAVIVLAVTSFSLVAAAQTLLPNWSHHPKARALRV
HLNQGLYVNALLDRALAPKPA