Protein Info for Dshi_0916 in Dinoroseobacter shibae DFL-12

Annotation: efflux transporter, RND family, MFP subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 34 to 366 (333 residues), 197.2 bits, see alignment E=1.7e-62 PF16576: HlyD_D23" amino acids 45 to 271 (227 residues), 61.7 bits, see alignment E=1.2e-20 PF13533: Biotin_lipoyl_2" amino acids 61 to 106 (46 residues), 26.8 bits, see alignment 7.2e-10 PF13437: HlyD_3" amino acids 184 to 275 (92 residues), 55.7 bits, see alignment E=1.4e-18

Best Hits

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_0916)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LRV4 at UniProt or InterPro

Protein Sequence (396 amino acids)

>Dshi_0916 efflux transporter, RND family, MFP subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MTLCSLLRPLYFMLALMVLPTVPPLFAQERPAAVVTETVEERALAETLPVFGQVVASRDS
AVASRTAGIVDEVAVLVGDRVAEGATLAVLDTELAQIELEQALADIAEARAGIAVAEAQL
GRAEDARDRVEGLRNTASFSSARFDDAMSDLAAATGELNQARARLLNAEAAEQRARYTLD
RARITAPFAGVVLSVAADPGQFISVGDPIARIIDINALEIEAQVPSQFAPAIPPGVTVKG
VSEAGQQLDLQIRAILPTESVSTRTRPVRLSGDLDALGATLAVGQSITLQVPVSAPRAAL
AVPKDALVQGAQGWTVFVDADGIAEPRPVRIGVAMGDRFEVLSGLAEGDAVVVRGNERLR
PGQALQAVDGAPGGGAPEEGARTPDDTQSRAAPQSN