Protein Info for Dshi_0911 in Dinoroseobacter shibae DFL-12

Annotation: Thiamine-phosphate diphosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02581: TMP-TENI
  • amino acids 14 to 181 (168 residues), 151.2 bits, E=1e-48

Best Hits

Swiss-Prot: 61% identical to THIE_RHIEC: Thiamine-phosphate synthase (thiE) from Rhizobium etli (strain CFN 42 / ATCC 51251)

KEGG orthology group: K00788, thiamine-phosphate pyrophosphorylase [EC: 2.5.1.3] (inferred from 100% identity to dsh:Dshi_0911)

MetaCyc: 43% identical to thiamine phosphate synthase (Escherichia coli K-12 substr. MG1655)
Thiamine-phosphate diphosphorylase. [EC: 2.5.1.3]; 2.5.1.3 [EC: 2.5.1.3]; 2.5.1.3 [EC: 2.5.1.3]

Predicted SEED Role

"Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
• thiamine diphosphate salvage IV (yeast) (5/7 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (2/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (2/3 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (1/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (1/2 steps found)
• thiamine diphosphate salvage II (3/5 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)
• thiamine diphosphate salvage V (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.3

Use Curated BLAST to search for 2.5.1.3

Sequence Analysis Tools

See A8LRU9 at UniProt or InterPro

Protein Sequence (199 amino acids)

>Dshi_0911 Thiamine-phosphate diphosphorylase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSALHPFYPIFDRAEWLIRLLPLGIKLVQLRVKDQPPEVIRAEIRAAKGLCAEHGCTLVV
NDHWQMAIAEGCDWVHLGQEDLDTADLGALRAAGIKLGISTHDEAELDRALACKPAYIAL
GPIYPTILKKMKWEQQGIEKLTEWKSRIGDVPLIAIGGMTPDRAPGALAAGADVVSAVTD
ITLNPDPEAQVRRWLEVLG