Protein Info for Dshi_0847 in Dinoroseobacter shibae DFL-12

Annotation: Dihydroorotate oxidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 11 to 347 (337 residues), 365.9 bits, E=8.4e-114
• PF01180: DHO_dh
  • amino acids 44 to 332 (289 residues), 253.6 bits, E=1.2e-79

Best Hits

Swiss-Prot: 74% identical to PYRD_RHOS1: Dihydroorotate dehydrogenase (quinone) (pyrD) from Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 100% identity to dsh:Dshi_0847)

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.98.1

Sequence Analysis Tools

See A8LRE2 at UniProt or InterPro

Protein Sequence (352 amino acids)

>Dshi_0847 Dihydroorotate oxidase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSTLERLGLAALQRVDPETAHGLALRALNAGLGPRSGPVTSPRLSTQLAGLRLPNPVGLA
AGFDKNAETLGALAQTGFGFLEVGAATPLPQPGNPRPRLFRLSEDRAAINRFGFNNDGAE
AIAARLARRPEGRVVGLNLGANKTSADRAGDFARVLATCGAHVDFATVNVSSPNTEKLRD
LQGAAALRALLEGVMAARAALVRPIPVFLKIAPDMDDAALDDIAGVVTEAGLHGIIATNT
TLARDGLVSAHKGEAGGLSGAPLFEASTRVLARLSQATEGTVPLIGVGGVDSAGAAYAKI
RAGASAVQLYTALVYGGISLAAEIATGLDTLLERDGFSTVADAVGTGRGDWL