Protein Info for Dshi_0820 in Dinoroseobacter shibae DFL-12

Annotation: alpha/beta hydrolase fold (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 PF00561: Abhydrolase_1" amino acids 14 to 166 (153 residues), 65.3 bits, see alignment E=1.7e-21 PF00975: Thioesterase" amino acids 15 to 96 (82 residues), 32 bits, see alignment E=3.6e-11 PF12146: Hydrolase_4" amino acids 16 to 120 (105 residues), 39.5 bits, see alignment E=1e-13 PF12697: Abhydrolase_6" amino acids 16 to 247 (232 residues), 79.9 bits, see alignment E=1.1e-25

Best Hits

KEGG orthology group: K01175, [EC: 3.1.-.-] (inferred from 100% identity to dsh:Dshi_0820)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LRB5 at UniProt or InterPro

Protein Sequence (255 amino acids)

>Dshi_0820 alpha/beta hydrolase fold (RefSeq) (Dinoroseobacter shibae DFL-12)
MLNTVIHGAATEAPPLLIVHGLFGSAKNWGAIAKRMAQHRQVLAVDLRNHGDSPHTQSHS
YPDLAADLAEVIAAHGGRADVLGHSMGGKAAMVLALSQPEMVARLLVADIAPVTYDRTQV
GLIDAMEGVDLSAVTKRSDADAALAADVPDAPTRAFLTQSLDLRAEGGPRWRLNLATLRR
DMPRIMGFPEIDGRYTGPTLFLRGGASDYVLPAHGAEIERLFPGADRDAIPDVGHWLHAE
APGPFIARLTDFLDA