Protein Info for Dshi_0795 in Dinoroseobacter shibae DFL-12
Annotation: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to HIS5_RUEPO: Imidazole glycerol phosphate synthase subunit HisH (hisH) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 100% identity to dsh:Dshi_0795)Predicted SEED Role
"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- L-histidine biosynthesis (10/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Purine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.4.2.-
Use Curated BLAST to search for 2.4.2.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A8LQZ7 at UniProt or InterPro
Protein Sequence (212 amino acids)
>Dshi_0795 imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (RefSeq) (Dinoroseobacter shibae DFL-12) MLTVLVDYDSGNLHSAEKAFERMAREVDGGEICVTSDPDLVARADRIVLPGDGAFPACRA GLEGFAGLFEAIEEAVTARARPFLGICVGMQLMATTGREYEDTPGFGWIDGEVVKITPSD PALKVPHMGWNDLVIDRPHPVLEGVATGDHAYFVHSYHMQVTDPATLLAHCDYGGPVTAV VGRDTMVGAQFHPEKSQAAGLRMIANFLTWRP