Protein Info for Dshi_0628 in Dinoroseobacter shibae DFL-12

Annotation: extracellular solute-binding protein family 1 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF12849: PBP_like_2" amino acids 25 to 254 (230 residues), 182.7 bits, see alignment E=1.3e-57 PF13531: SBP_bac_11" amino acids 42 to 261 (220 residues), 49.5 bits, see alignment E=5.1e-17

Best Hits

KEGG orthology group: K05772, putative tungstate transport system substrate-binding protein (inferred from 100% identity to dsh:Dshi_0628)

Predicted SEED Role

"ABC-type tungstate transport system, periplasmic binding protein" in subsystem ABC transporter tungstate (TC 3.A.1.6.2)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQ88 at UniProt or InterPro

Protein Sequence (276 amino acids)

>Dshi_0628 extracellular solute-binding protein family 1 (RefSeq) (Dinoroseobacter shibae DFL-12)
MRLALISSLFAACLGAATTLAGVAQASEPSILVQSTTSTQNSGLFDHILPIFEAETGIDV
QVVAVGTGQALRNAANGDGDVLFVHAKAAEEKFVADGFGVSRADVMYNDFVIVGPAADPA
GVGGSSDAVAALAQIAEAQAPFLSRGDDSGTNKAELRLWSETGIDAQAASGDWYRETGSG
MGATLNTGIAMNGYVMTDRATWIAFGNKQNHEILVEGDPKLFNQYGIILVNPERHAHVKA
DLGQQFVDWILSEEGQAAIASFKVDGQQLFFPNAGG