Protein Info for Dshi_0617 in Dinoroseobacter shibae DFL-12

Annotation: ATPase AAA-2 domain protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 871 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 850 (845 residues), 1368.8 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 68 (52 residues), 46.7 bits, see alignment 1.7e-15 amino acids 94 to 144 (51 residues), 21.3 bits, see alignment 1.5e-07 PF00004: AAA" amino acids 203 to 315 (113 residues), 44.3 bits, see alignment E=1.5e-14 amino acids 600 to 716 (117 residues), 32.3 bits, see alignment E=7.5e-11 PF17871: AAA_lid_9" amino acids 342 to 444 (103 residues), 120.3 bits, see alignment E=1.9e-38 PF07724: AAA_2" amino acids 594 to 757 (164 residues), 228.9 bits, see alignment E=2.2e-71 PF07728: AAA_5" amino acids 599 to 717 (119 residues), 45.8 bits, see alignment E=3.9e-15 PF10431: ClpB_D2-small" amino acids 764 to 843 (80 residues), 99.9 bits, see alignment E=3.9e-32

Best Hits

Swiss-Prot: 73% identical to CLPB_BRUSU: Chaperone protein ClpB (clpB) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 73% identity to bcs:BCAN_A1908)

MetaCyc: 57% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LPP0 at UniProt or InterPro

Protein Sequence (871 amino acids)

>Dshi_0617 ATPase AAA-2 domain protein (RefSeq) (Dinoroseobacter shibae DFL-12)
MNMEKFTERSRGFLQAAQTIAMRENHQRMVPEHLLKALLDDEEGLAANLIARAGGDAARV
REALAAALDKLPKVSGDGAQTYLDQQTAKVLDEAEKLATKAGDSFVPVERILTALAVTKS
GAKTALEAGGVSAQALNGAINDIRKGRTADTASAESGYDALKKFARDLTEAAADGKIDPI
IGRDEEIRRAMQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIINGDVPESLRNKKLMAL
DMGSLIAGAKYRGEFEERLKSVLSEVTAAAGEIILFIDEMHTLVGAGKADGAMDASNLLK
PALARGELHCVGATTLDEYRKYVEKDAALARRFQPVMVQEPTVEDTVSILRGIKEKYELH
HGVRISDSALVAAATLSHRYITDRFLPDKAIDLVDEAASRLRMEVDSKPEELDALDREIL
QKQIEAEALRLEDDAASRERLASLEKDLSDLQQRSAEMTAQWQSERDKLASARDLKEHLD
KARAELEIAKREGNLARAGELSYGVIPGLEKQLAEAESGEGDVMVEEAVRPEQIASVVER
WTGIPTSKMLEGEREKLLRMEEEIGRRVIGQKTAVNAVANAVRRARAGLNDENRPLGSFL
FLGPTGVGKTELTKAVAEFLFDDDSAMVRIDMSEFMEKHAVARLIGAPPGYVGYDEGGVL
TEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGVLTDGQGRTVDFKQTLIVLTSNLGA
QALSQLPDGADASAAKRDVMDAVRAHFRPEFLNRLDETIIFDRLVRDDMDAIVDIQLARL
QKRLDARKLTLALDDAARSWLADEGYDPVFGARPLKRVIQRALQDQLAEMILAGDVTDGE
TIPVSAGPDGLLVGDRVSTSRRVPPQDAVVH