Protein Info for Dshi_0577 in Dinoroseobacter shibae DFL-12

Annotation: aldehyde dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 PF00171: Aldedh" amino acids 13 to 474 (462 residues), 526.4 bits, see alignment E=2.8e-162

Best Hits

Swiss-Prot: 37% identical to BETB_BACSU: Betaine aldehyde dehydrogenase (gbsA) from Bacillus subtilis (strain 168)

KEGG orthology group: K00128, aldehyde dehydrogenase (NAD+) [EC: 1.2.1.3] (inferred from 100% identity to dsh:Dshi_0577)

MetaCyc: 52% identical to 2,5-dioxovalerate dehydrogenase (Caulobacter vibrioides CB15)
2,5-dioxovalerate dehydrogenase. [EC: 1.2.1.26]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.22, 1.2.1.26, 1.2.1.3

Use Curated BLAST to search for 1.2.1.22 or 1.2.1.26 or 1.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LPK0 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Dshi_0577 aldehyde dehydrogenase (RefSeq) (Dinoroseobacter shibae DFL-12)
MTDRTQLYIDGAWTEGTAQIENRNPSDTTDLIGMYAQADAGQLDTALAAARRAQPAWWAA
GIQKRHDVLMAIGTELMARSDEIGRLLSREEGKPLAEGKGEVYRAGQFFTYFAAEALRQH
GDLAESVRPGIEIDVRREAVGVVAIISPWNFPVATPAWKIAPALAFGNAVVWKPANVTPA
SAIALTEIIARQDIPKGLFNLVAGPGRDVGQRLVESAEVDAISFTGSVPVGRGIAAAAVQ
NMTKVQMEMGSKNPLIVMDDCDLDLAVAHAASSAFGGTGQKCTAASRLIVHSAVHDAFVE
KLVAAARAMKVGHALEDGTQLGPVVSESQLNQNMEYIGVGKDEGAELLCGGDRLEMATDG
YFMAPAVFAGTANDMRINREEMFAPITAVQRVDSYDEALARANDTQFGLTAGIMTTSLAR
ASHFRAHMRAGCVMVNLPTAGTDYHVPFGGRGASSFGPREQGSYAAEFYTTVKTAYVAAG
APA