Protein Info for Dshi_0558 in Dinoroseobacter shibae DFL-12

Annotation: D-amino-acid dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF01266: DAO" amino acids 2 to 398 (397 residues), 252.8 bits, see alignment E=3.4e-78 PF01494: FAD_binding_3" amino acids 2 to 33 (32 residues), 22.1 bits, see alignment (E = 3.8e-08) PF01946: Thi4" amino acids 2 to 36 (35 residues), 26.4 bits, see alignment 1.7e-09 PF00070: Pyr_redox" amino acids 2 to 33 (32 residues), 23.5 bits, see alignment (E = 3e-08) PF13450: NAD_binding_8" amino acids 5 to 44 (40 residues), 30.4 bits, see alignment 1.7e-10

Best Hits

Swiss-Prot: 58% identical to DADA_METNO: D-amino acid dehydrogenase (dadA) from Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to dsh:Dshi_0558)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LPI1 at UniProt or InterPro

Protein Sequence (419 amino acids)

>Dshi_0558 D-amino-acid dehydrogenase (RefSeq) (Dinoroseobacter shibae DFL-12)
MKVVIMGAGVIGVTTAYYLAKQGVKVVVIDRQTGPGLETSYANAGQLSYGMSSPWAAPGI
PLKAVKWMFMKRRPLFIMPLLSPTMWKWCLQMLRNCNAESYAINKGRMVRVSSYSRDVMP
DLIADTGIEYDGRQMGTLQLFRTAKQMKASKADQAVLAEYGSPYEVLGRDACIEVEPALA
EVRGKFVGGLRLTSDRTGDCRMFTIALTDKCIEMGVEFQYGQTIKAIAVENGKISGVDTE
IAGRISGDAYVCAMGSYAVNVLNPIGIRLPVYPVKGYSVTLPVTNDAFAPQSTIMDETHK
VAITRLGDRIRVAGQAEIVGYSNRLGPHATDTVRHVIGDLFPKGGDLSRAEGWTGLRPMT
PDGTPVLGPTRYENLFLNTGHGTLGWTMACGSGRAVADVVMGKPPEISMEGLTAARYAR