Protein Info for Dshi_0531 in Dinoroseobacter shibae DFL-12

Annotation: ABC sugar transporter, periplasmic ligand binding protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF13407: Peripla_BP_4" amino acids 32 to 289 (258 residues), 227 bits, see alignment E=3e-71 PF00532: Peripla_BP_1" amino acids 33 to 239 (207 residues), 22.9 bits, see alignment E=5.4e-09

Best Hits

KEGG orthology group: K02058, simple sugar transport system substrate-binding protein (inferred from 100% identity to dsh:Dshi_0531)

Predicted SEED Role

"Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1)" in subsystem D-ribose utilization (TC 3.A.1.2.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LNW9 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Dshi_0531 ABC sugar transporter, periplasmic ligand binding protein (RefSeq) (Dinoroseobacter shibae DFL-12)
MKTKFTLLASVAMASSALFATQAVADGHSKDIATVVKIAGIQWFNRMEEGVKKFAEETGM
NAFQVGPAQADPQQQVALIEDMIAQGVDALAVVPMSPEALEPVLGRAMEAGITVITHEAA
AQQNTTYDLEAFVNEDFGANLMEQLATCMGGEGEYAVFVGSLTSQTHNQWVDGAIAYQEA
NYPNMTLVGDKNETFDDAEQAYTKTQEVLRAFPNIKGMQGSASTDVAGIGRAIEERGMED
ATCVFGTSLPSIAGQYLETGAVDGIGFWDPAVAGEAMNKLAVMVMNGEEVTDGMDLGLPG
YESVSLDGKVIYGQAWVNVDAENMSEYPF