Protein Info for Dshi_0504 in Dinoroseobacter shibae DFL-12

Annotation: molybdopterin oxidoreductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 986 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF04879: Molybdop_Fe4S4" amino acids 72 to 121 (50 residues), 45.9 bits, see alignment 6.9e-16 PF00384: Molybdopterin" amino acids 125 to 583 (459 residues), 163.9 bits, see alignment E=8.6e-52 PF01568: Molydop_binding" amino acids 852 to 979 (128 residues), 84.3 bits, see alignment E=9.1e-28

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to dsh:Dshi_0504)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LNU4 at UniProt or InterPro

Protein Sequence (986 amino acids)

>Dshi_0504 molybdopterin oxidoreductase (RefSeq) (Dinoroseobacter shibae DFL-12)
MLVKRRSTDARRSGLAAFAQNVSAKPVDRRSFLRSSGLAVGGLAALGTVGATTVRRAEAG
MTDFSQPVEIKTNICTHCSVGCTVLAEVQNGVWVGQEPAWKSPINRGTHCAKGASVRELV
HGDRRLKYPMKKENGAWVRISWDQAIDEIGDKMLDIREKHGADSAYLLGSAKFSNEGAYL
FRKFAAFWGTNNVDHQARICHSTTVAGVANTWGYGAMTNSYNDIRNSKTIIFMGSNAAEA
HPVSLQHILTGKETNRANVIVIDPRFTRTAAHATDYIRMRPGTDIAVIWGIMWHIFENNW
QDQEYIDQRVWGMDQIREAVKAYPPAEVERITGIGEAELRRVAETFATQGPATFIWCMGG
TQHTVGTANVRTYCNLLLATGNVGSNGSGANIFRGHCNVQGATDFGLDIGSLPCYYGLSE
GGWRHWSRVWEVDYDYLQARFDEVPAKGGRDARDRKANMEMAGIPSTRWFDATTLDAEDV
DQRDNIKAMIVFGHGGNTVPRMGDSLKGMNALDLLVVADPHPTTFAALHDRPDNTYLLPI
STQFECDGSRTASNRSVQWGEKVVEPIFESDNDYAVIYRLAEKLGFADEMFKNYEMVQGK
FSMEPTAESVLREINRGGWSTGYTGQSPERLKAHMANQDKFDLVTLRAPADAGDVAGDFY
GLPWPCWGTPEFKHPGTHILYNPNVPVKEGGGGFRPRFGLDRDGETLLAEGSYPVGSEIE
DGYPQFTYGILQSLGWDSDLTAEELATIQRIGGNDPEAMANVSWSLDLSGGIQRVAIEHG
CVPFGNGKARAVAWNLPDPIPVHREPIYSPRPDLVADYPTRDDGRQFRVPNIGHTMQASA
VERNLSESFPIVLTSGRLVEYEGGGEETRSNPWLAELQQDMFVEINPADAEARGISDGGW
VWVYGPENESKTRVKALVTERVAEGVAFMPFHFAGWFQGEDQRGNYPEGTDPIVLGESVN
TVTSYGYDPVTGMHEGKVTLCQIAAA