Protein Info for Dshi_0451 in Dinoroseobacter shibae DFL-12

Annotation: coenzyme PQQ biosynthesis protein B (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 TIGR02108: coenzyme PQQ biosynthesis protein B" amino acids 6 to 296 (291 residues), 371.4 bits, see alignment E=1.5e-115 PF12706: Lactamase_B_2" amino acids 51 to 265 (215 residues), 132.9 bits, see alignment E=1.2e-42 PF00753: Lactamase_B" amino acids 66 to 166 (101 residues), 23 bits, see alignment E=7e-09

Best Hits

Swiss-Prot: 62% identical to PQQB_RUEPO: Coenzyme PQQ synthesis protein B (pqqB) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K06136, pyrroloquinoline quinone biosynthesis protein B (inferred from 100% identity to dsh:Dshi_0451)

Predicted SEED Role

"Coenzyme PQQ synthesis protein B" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LN55 at UniProt or InterPro

Protein Sequence (297 amino acids)

>Dshi_0451 coenzyme PQQ biosynthesis protein B (RefSeq) (Dinoroseobacter shibae DFL-12)
MAFRAHILGAAAGGGLPQWNCGCDNCNLARSGTIPPQTQSSLAVTADGTSWAILNASPDI
RGQLADTPALHPTGLRDLPLRAVLVTNGDIDHVAGLLTLREMQPFRLFATAGIHEVLAEN
PIFNAVNATVVPRETIALDAPFELVPGLIATLFPVPGKVPLYLEGETVETDLIGDQTVGV
RLQALDSTAYYIPGCALLTDDLRARLDGADTLFFDGTLWADDEMVRAGLGKKTGKRMGHM
SMSGPDGSIAAFEGLSIRQKIYVHMNNTNPVLRPSSAEKAQAEAAGWIIGQDGMEVS