Protein Info for Dshi_0402 in Dinoroseobacter shibae DFL-12

Annotation: CRISPR-associated protein Cas2 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 114 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details TIGR01573: CRISPR-associated endonuclease Cas2" amino acids 14 to 104 (91 residues), 74.6 bits, see alignment E=2.9e-25 PF09827: CRISPR_Cas2" amino acids 14 to 92 (79 residues), 58.3 bits, see alignment E=3.7e-20

Best Hits

Swiss-Prot: 50% identical to CAS2_ACIAP: CRISPR-associated endoribonuclease Cas2 (cas2) from Acidovorax avenae (strain ATCC 19860 / DSM 7227 / JCM 20985 / NCPPB 1011)

KEGG orthology group: K09951, CRISPR-associated protein Cas2 (inferred from 100% identity to dsh:Dshi_0402)

Predicted SEED Role

"CRISPR-associated protein Cas2" in subsystem CRISPRs

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LN07 at UniProt or InterPro

Protein Sequence (114 amino acids)

>Dshi_0402 CRISPR-associated protein Cas2 (RefSeq) (Dinoroseobacter shibae DFL-12)
MTDAPAFLSGYRIMWILVMFDLPTDTKSQRKAATAFRNFLLDEGFERSQFSVYARFVNGK
EAFQTRVRRIERNLPEKGDIQVLNFTDRQYRDIVHFSDQGRRAARKNPEQLALF