Protein Info for Dshi_0320 in Dinoroseobacter shibae DFL-12

Annotation: polar amino acid ABC transporter, inner membrane subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 transmembrane" amino acids 36 to 63 (28 residues), see Phobius details amino acids 109 to 128 (20 residues), see Phobius details amino acids 135 to 151 (17 residues), see Phobius details amino acids 156 to 172 (17 residues), see Phobius details amino acids 180 to 202 (23 residues), see Phobius details amino acids 222 to 248 (27 residues), see Phobius details amino acids 260 to 288 (29 residues), see Phobius details amino acids 296 to 316 (21 residues), see Phobius details amino acids 353 to 378 (26 residues), see Phobius details amino acids 396 to 417 (22 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 221 to 318 (98 residues), 49 bits, see alignment E=3.5e-17 PF00528: BPD_transp_1" amino acids 240 to 401 (162 residues), 57.8 bits, see alignment E=6.1e-20

Best Hits

KEGG orthology group: K09971, general L-amino acid transport system permease protein (inferred from 100% identity to dsh:Dshi_0320)

Predicted SEED Role

"Glutamate transport membrane-spanning protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LM92 at UniProt or InterPro

Protein Sequence (432 amino acids)

>Dshi_0320 polar amino acid ABC transporter, inner membrane subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MSDTHASAAFVRTEEIPPSPPPVSEVGIVNWLRSNLFYSIPSSILTLLTAAFIYWLSSIL
MPWMFNGVWDANSIRDCREILDGAIGGCFAVIAERWPQLIFGNYPNEEYWRAVLAFVGLF
VALAPVLFFKLPRKMLWFTGLFPFIAYWLIWGGSAWVSVMALVGVVVGYVAFKALEERSF
FGAIVAALFATLLFWIFISPLAVNALSSVVALSLPQVESRFIGGFMINFILGTSGIALSL
PLGIALALGRQSNLPIIKGVCVVFIEFIRGVPLITLLFVASVMLAYFLPPGTSFDLVLRV
IIMITLFASAYIAEAIRGGLAALPRGQYEAGDSLGLDYWQSMRLIILPQALKISIPSIVN
IAIGLFKDTTLVSIISMFDMLGMIQGPILSSTEWFGVYWELLGFAGVLFFVFCYGISQYS
QWLERQLATEHR