Protein Info for Dshi_0253 in Dinoroseobacter shibae DFL-12

Annotation: AMP-dependent synthetase and ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 PF00501: AMP-binding" amino acids 26 to 376 (351 residues), 297.4 bits, see alignment E=1.4e-92 PF13193: AMP-binding_C" amino acids 427 to 501 (75 residues), 74.4 bits, see alignment E=1.1e-24

Best Hits

KEGG orthology group: None (inferred from 100% identity to dsh:Dshi_0253)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LLJ2 at UniProt or InterPro

Protein Sequence (519 amino acids)

>Dshi_0253 AMP-dependent synthetase and ligase (RefSeq) (Dinoroseobacter shibae DFL-12)
MKIYRSPFADVTVRDLSITEALFEGLARRGDAPILIDGPSGAAMTGAQLEGRIRACAGGL
RARGIGPGDVVAIMAPNMPDYATAFHGAAFAGATVTTLNPTYTTEEAAHQLRDSGAQMLV
TVPAFADLAAEAVQGTGVTETVMMGTTGPGSLEALFGPPLAAQVAVDLARDIVVLPYSSG
TTGLPKGVMLSHRNLVVNVDQTAEIIGITVQDVTVGFLPFFHIYGMTVLMNCYLSRGAAV
VTMPRFDLEQFLSLCQTHRPRQLYIAPPVALALAKHPMVDDYDLSGVEFILSGAAPLGGD
VAEAVGRRLGVEMVQGYGMTEMSPVSHFTPPGQNVPGSVGPTAPSAESRIVDPETGEDAA
EGEVWVRGPQIMQGYLNRPDATAETVTRDGWLKTGDLGRFDEAGNLFITDRVKELIKVSG
FQVAPAELEAVLLTHPAITDAAVIGVPDDSAGERPMAFVVRSDPDLSEGAVIAHAAEHLA
HYKRIARVAFVEAVPKSASGKILRRLLRAKVGEDMATGA